Increase in ovarian blood volume during ovulation in the gonadotropin-primed immature rat.
Increase in ovarian blood volume during ovulation in the gonadotropin-primed immature rat.
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促性腺激素引发的未成熟大鼠排卵期间卵巢血量增加。
DOI：
10.1095/biolreprod40.4.762
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发表时间：
1989
影响因子：
3.6
通讯作者：
Okamura,H
中科院分区：
文献类型：
--
作者：
Tanaka,N;Espey,LL;Okamura,H
本研究通过测量卵巢提取物中血红蛋白的光密度（414 nm），并将该测量结果与已知量全血的光密度进行比较，来定量Wistar大鼠的卵巢血容量。未成熟大鼠用孕马血清促性腺激素（PMSG），10 IU s.c.，在23天的年龄。第25天，通过人绒毛膜促性腺激素（hCG）10 IU s.c.启动排卵过程，10 h后输卵管内开始出现卵子。每隔2小时，摘除卵巢，并在1.0 ml 0.05 M三（羟甲基）氨基甲烷缓冲液（pH 7.4）中匀浆30 s。将匀浆离心20 min，并用吉尔福德RESPONSE UV/维斯分光光度计分析上清液。这些卵巢提取物中的血红蛋白具有与通过大鼠心脏穿刺获取的全血中的氧合血红蛋白相同的414 nm特征峰吸光度。吸光度与样品中全血体积呈线性关系。8只大鼠hCG后0 h每个卵巢的血量为0.60 ± 0.07 μl。hCG后4 h体积增至1.37 ± 0.26 μl，10 h达高峰，为4.55 ± 0.72 μl。吲哚美辛治疗（0.3 - 10.0 mg/大鼠，s.c.）部分抑制卵巢血容量增加7倍。总之，排卵期卵巢血容量的增加可能反映了炎症组织特有的血管扩张和充血。
This study quantifies ovarian blood volume in Wistar rats by measuring the optical density (414 nm) of hemoglobin in ovarian extracts and comparing this measurement to the optical density of known amounts of whole blood. Immature rats were primed with pregnant mare’s serum gonadotropin (PMSG), 10 IU s.c., at 23 days of age. On Day 25, the ovulatory process was initiated by human chorionic gonadotropin (hCG), 10 IU s.c., and ova began to appear in the oviducts 10 h later. At 2-h intervals, the ovaries were extirpated and homogenized in 1.0 ml of 0.05 M tris (hydroxymethyl)aminomethane buffer (pH 7.4) for 30 s. Homogenates were centrifuged for 20 min and the supernatant fluids were analyzed with a Gilford RESPONSE UV/VIS spectrophotometer. The hemoglobin in these ovarian extracts had the same peak absorbance of 414 nm characteristic of oxyhemoglobin in whole blood taken by cardiac puncture of the rats. There was a linear relationship between the absorbance and the volume of whole blood in the samples. The volume of blood per ovary from groups of 8 rats was 0.60 ± 0.07 μl at 0 h after hCG. The volume increased to 1.37 ± 0.26 μl at 4 h after hCG and reached a peak of 4.55 ± 0.72 μl at 10 h. Indomethacin treatment (0.3−10.0 mg/rat, s.c.) partially inhibited this 7-fold increase in ovarian blood volume. In conclusion, the increase in ovarian blood volume during ovulation may reflect the vasodilation and hyperemia that are characteristic of inflamed tissues.