Temporal requirement of C/EBPβ in the amygdala following reactivation but not acquisition of inhibitory avoidance
Temporal requirement of C/EBPβ in the amygdala following reactivation but not acquisition of inhibitory avoidance
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DOI：
10.1101/lm.598307
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发表时间：
2007-07-01
影响因子：
2
通讯作者：
Alberini, Cristina M.
中科院分区：
文献类型：
--
作者：
Milekic, Maria H.;Pollonini, Gabriella;Alberini, Cristina M.
在学习之后，记忆是脆弱的，为了变得稳定，需要经历一个依赖蛋白质合成的巩固过程。如果重新激活，已建立的记忆可能会再次对中断变得短暂敏感，并需要另一个依赖蛋白质合成的过程，称为再巩固，以维持记忆。在这里，我们发现，在基底外侧杏仁核（BLA）中，蛋白质合成对于抑制避免（IA）记忆的巩固和再巩固都是必要的，而转录因子CCAAT增强子结合蛋白β (C/EBP β)的表达仅对于再巩固过程是必要的。此外，蛋白质合成和C/ EBP β在IA再激活后的关键作用暂时受到限制，因为它们仅对最近的IA记忆是必要的，而不是旧的IA记忆。这些结果，加上之前的研究结果表明，在海马体中，蛋白质合成和C/EBP β表达是IA巩固而不是IA再巩固所必需的，这表明在训练或记忆检索后发生的稳定过程涉及不同的神经回路。在这些回路中，C/EBP β依赖的分子通路似乎被不同地募集。
Following learning, a memory is fragile and undergoes a protein synthesis- dependent consolidation process in order to become stable. Established memories can again become transiently sensitive to disruption if reactivated and require another protein synthesis-dependent process, known as reconsolidation, in order to persist. Here, we show that, in the basolateral amygdala (BLA), protein synthesis is necessary for both consolidation and reconsolidation of inhibitory avoidance (IA) memory, while the expression of the transcription factor CCAAT enhancer binding protein beta (C/EBP beta) is essential only for the reconsolidation process. Moreover, the critical roles of both protein synthesis and C/ EBP beta following IA reactivation are temporally restricted, as they are necessary only for recent but not old IA memories. These results, together with previous findings showing that in the hippocampus both protein synthesis and C/EBP beta expression are required for consolidation but not reconsolidation of IA indicate that the stabilization process that takes place either after training or memory retrieval engages distinct neural circuits. Within these circuits, the C/EBP beta-dependent molecular pathway appears to be differentially recruited.