METHYLATED REGIONS OF HAMSTER MITOCHONDRIAL RIBOSOMAL-RNA - STRUCTURAL AND FUNCTIONAL CORRELATES
METHYLATED REGIONS OF HAMSTER MITOCHONDRIAL RIBOSOMAL-RNA - STRUCTURAL AND FUNCTIONAL CORRELATES
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DOI：
10.1093/nar/9.2.323
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
DUBIN, DT
中科院分区：
文献类型：
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作者：
BAER, RJ;DUBIN, DT
仓鼠线粒体核糖体RNA中转录后甲基化残基的位置已经确定。与其他线粒体rRNA以及与细菌、真核生物和叶绿体rRNA的比较表明，甲基化区域i)由保守的一级序列和/或二级结构组成，ii)位于核糖体的亚基界面。对比分析还表明，仓鼠线粒体大核糖体亚基（LSU1） RNA的核糖甲基化序列UmGmU位于一个普遍保守的发夹环中，该发夹环含有一个推定的嘌呤霉素反应核苷酸。“UmGmU发夹”位于LSU RNA的两个氯霉素耐药残基的100个核苷酸内。我们提出了这个区域的二级结构，它在LSU rna中是保守的。这种结构允许三个抗生素相互作用位点的物理并置，从而定义了3 '端氨基基- trna的核糖体结合位点的RNA成分。
The positions of post-transcriptionally methylated residues within hamster mitochondrial ribosomal RNA have been established. Comparisons with other mitochcndrial rRNA, and with bacterial, eucaryotic and chloroplast rRNA show that the methylated regions i) are comprised of conserved primary sequences and/or secondary structures and ii) are situated at the subunit interface of the ribosome.The comparative analyses also reveal that the ribose-methylated sequence UmGmU of hamster mitochondrial large ribosomal subunit (LSU1) RNA lies in a universally conserved hairpin loop which contains a putative puromycin-reactive nucleotide. The “UmGmU hairpin” is within 100 nucleotides of two chloramphenicol-resistance residues of LSU RNA. We present a secondary structure for this region which is conserved in LSU RNAs. This structure allows physical juxtaposition of the three antibiotic-interacting loci and thus defines RNA components of the ribosomal-binding site for the 3′-termlnusof aminoacyl-tRNA.