Genome-Wide Identification and Characterization of WRKY Gene Family in Peanut.
Genome-Wide Identification and Characterization of WRKY Gene Family in Peanut.
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DOI：
10.3389/fpls.2016.00534
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发表时间：
2016
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Song H;Wang P;Lin JY;Zhao C;Bi Y;Wang X
WRKY是一个重要的转录因子家族，广泛分布于植物界。WRKY蛋白在不同植物中的系统发育关系和生物学功能的研究报道较多。然而，对花生属植物基因组中WRKY蛋白及其对水杨酸（SA）和茉莉酸（JA）处理的反应知之甚少。在这项研究中，我们确定了77和75 WRKY蛋白从两个野生祖先二倍体基因组的栽培四倍体花生，Arachis duranensis和Arachis ipaënsis，使用生物信息学方法。多数花生WRKY编码基因位于A. duranensis染色体A6和A. ipaënsis B3染色体上的WRKY基因数目最多，而9号染色体上的WRKY基因数目最少。WRKY基因在A. duranensis和A. Ipaënsis染色体高度同线。我们的分析表明，节段重复事件在AdWRKY和AiWRKY基因中起主要作用，并且在基因重复对中观察到强烈的纯化选择。此外，我们从野生祖先花生的全基因组分析结果中获得的知识转化到栽培花生，揭示了特定栽培花生WRKY基因的基因活性由于SA和JA处理而改变。花生WRKY7、8和13基因在SA和JA处理下表达下调，而WRKY1和12基因在SA和JA处理下表达上调。这些结果可为花生品种改良提供有价值的信息。
WRKY, an important transcription factor family, is widely distributed in the plant kingdom. Many reports focused on analysis of phylogenetic relationship and biological function of WRKY protein at the whole genome level in different plant species. However, little is known about WRKY proteins in the genome of Arachis species and their response to salicylic acid (SA) and jasmonic acid (JA) treatment. In this study, we identified 77 and 75 WRKY proteins from the two wild ancestral diploid genomes of cultivated tetraploid peanut, Arachis duranensis and Arachis ipaënsis, using bioinformatics approaches. Most peanut WRKY coding genes were located on A. duranensis chromosome A6 and A. ipaënsis chromosome B3, while the least number of WRKY genes was found in chromosome 9. The WRKY orthologous gene pairs in A. duranensis and A. ipaënsis chromosomes were highly syntenic. Our analysis indicated that segmental duplication events played a major role in AdWRKY and AiWRKY genes, and strong purifying selection was observed in gene duplication pairs. Furthermore, we translate the knowledge gained from the genome-wide analysis result of wild ancestral peanut to cultivated peanut to reveal that gene activities of specific cultivated peanut WRKY gene were changed due to SA and JA treatment. Peanut WRKY7, 8 and 13 genes were down-regulated, whereas WRKY1 and 12 genes were up-regulated with SA and JA treatment. These results could provide valuable information for peanut improvement.