Characterization of allergens of Anisakis simplex
Characterization of allergens of Anisakis simplex
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DOI：
10.1046/j.1398-9995.2003.00344.x
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发表时间：
2003-12-01
期刊：
影响因子：
12.4
通讯作者：
Fernández-Caldas, E
中科院分区：
文献类型：
--
作者：
Arlian, LG;Morgan, MS;Fernández-Caldas, E
背景：单纯异尖线虫是一种海洋哺乳动物肠道寄生虫。幼虫会感染甲壳类动物、头足类动物和鱼类。人类在食用受感染的生鱼或未煮熟的鱼时，可摄入单纯单胞杆菌第三期幼虫（L3）。当幼虫穿透消化粘膜时，会引起炎症反应。幼虫或其分泌/排泄产物可使人致敏并诱导免疫球蛋白E （IgE）介导的过敏反应。这种寄生虫现在与许多吃鱼后过敏反应的病例有关。本研究的目的是评估第三期幼虫提取物中存在的蛋白质的致敏性。方法：采用交叉免疫电泳（CIE）法，将纯化成单纯a体细胞提取物（L3）的兔抗血清与同一体细胞提取物进行反应。采用交叉放射免疫电泳（cre）方法，先将CIE凝胶培养于13例单纯弧菌免疫cap阳性患者的血清中，然后再培养于放射标记的抗人IgE中。结果：体细胞提取物与体细胞抗血清反应后，在考马斯蓝染色的CIE凝胶上可见12 ~ 16个抗原抗体沉淀峰。CRIE凝胶放射自显影显示患者血清中有18种不同的蛋白结合IgE。个别患者的血清IgE针对2到10种不同的过敏原。其中五种过敏原被大于或等于50%的患者识别。所有患者均未识别出过敏原，且没有患者血清中有针对全部18种过敏原的IgE。结论：单纯弓形虫L3幼虫体提取物中含有大量致敏分子，不同患者对这些致敏原的敏感性和反应性存在显著差异。
Background: Anisakis simplex is an intestinal parasite of sea mammals. The larvae infect crustaceans, cephalopods and fish. Humans may consume A. simplex third stage larvae (L3) when eating infected raw or under-cooked fish. Consumed larvae cause an inflammatory reaction when they penetrate the digestive mucosa. The larvae or their secretory/excretory products can sensitize humans and induce an immunoglobulin E (IgE)-mediated allergic reaction. This parasite is now being implicated in numerous cases of allergic reactions after eating fish. The purpose of this study was to evaluate the allergenicity of proteins present in an extract of the third stage larva.Methods: Rabbit antiserum raised to A. simplex somatic extract (L3) was reacted by crossed immunoelectrophoresis (CIE) with the same somatic extract. Crossed radioimmunoelectrophoresis (CRIE) was also performed by incubating CIE gels first in the sera of 13 individuals with positive immunoCAP to A. simplex and then in radiolabeled anti-human IgE.Results: Twelve to 16 antigen-antibody precipitin peaks were visualized on Coomassie blue stained CIE gels in which somatic extract was reacted with somatic-antiserum. Autoradiography of CRIE gels showed that 18 different proteins bound IgE in patient sera. Individual patients had serum IgE directed at two to 10 different allergens. Five of these allergens were recognized by greater than or equal to50% of the patients. No allergen was recognized by every patient and no patient had serum IgE directed at all 18 allergens.Conclusion: Somatic extracts of A. simplex L3 larva contain a large number of allergenic molecules and there is significant variability between patients in their sensitivity and reactivity to these allergens.