Translational value of mechanical and vasomotor properties of mouse isolated mesenteric resistance-sized arteries.
Translational value of mechanical and vasomotor properties of mouse isolated mesenteric resistance-sized arteries.
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DOI：
10.1002/prp2.200
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发表时间：
2015-12
影响因子：
2.6
通讯作者：
Sheykhzade M
中科院分区：
文献类型：
--
作者：
Outzen EM;Zaki M;Abdolalizadeh B;Sams A;Boonen HC;Sheykhzade M
小鼠越来越多地用于血管研究，用于研究小动脉制剂中血管活性剂的扰动和反应。历史上，最好使用钢丝肌描记技术在大鼠离体肠系膜阻力大小动脉（MRA）中研究小动脉功能。虽然不同的小鼠动脉已经研究了使用钢丝肌描记器没有建立最佳设置尚未执行。因此，本研究的目的是首先建立小鼠MRA的钢丝肌电研究的最佳设置，并将其与大鼠MRA进行比较。其次，通过调查文献，我们的目的是评估整体的可翻译性观察到的药理学血管反应的小鼠MRA大鼠MRA以及相应的和不同的动脉血管的大小和物种起源。我们的研究结果表明，在小鼠MRA的最大主动力发展的最佳条件没有显着不同的大鼠MRA中确定的。此外，我们发现小鼠MRA对去甲肾上腺素、苯肾上腺素、血管紧张素II、sarafotoxin 6c、5-羟色胺、卡巴胆碱、硝普钠和瑞替加滨的浓度依赖性血管舒张反应与大鼠MRA以及不同大小和种属来源的动脉中描述的反应基本相似。总之，本研究的结果提供了一个基于证据的框架，用于优化小鼠MRA的等长钢丝肌描记器设置。此外，在翻译价值方面，我们的研究表明，小鼠MRA可以作为一个有用的模型，用于研究血管反应性。
Mice are increasingly used in vascular research for studying perturbations and responses to vasoactive agents in small artery preparations. Historically, small artery function has preferably been studied in rat isolated mesenteric resistance‐sized arteries (MRA) using the wire myograph technique. Although different mouse arteries have been studied using the wire myograph no establishment of optimal settings has yet been performed. Therefore, the purposes of this study were firstly to establish the optimal settings for wire myograph studies of mouse MRA and compare them to those of rat MRA. Second, by surveying the literature, we aimed to evaluate the overall translatability of observed pharmacological vasomotor responses of mouse MRA to those obtained in rat MRA as well as corresponding and different arteries in terms of vessel size and species origin. Our results showed that the optimal conditions for maximal active force development in mouse MRA were not significantly different to those determined in rat MRA. Furthermore, we found that the observed concentration‐dependent vasomotor responses of mouse MRA to noradrenaline, phenylephrine, angiotensin II, sarafotoxin 6c, 5‐hydroxytryptamine, carbachol, sodium nitroprusside, and retigabine were generally similar to those described in rat MRA as well as arteries of different sizes and species origin. In summary, the results of this study provide a framework for evidence‐based optimization of the isometric wire myograph setup to mouse MRA. Additionally, in terms of translational value, our study suggests that mouse MRA can be applied as a useful model for studying vascular reactivity.