Neuropeptides degranulate serous cells of ferret tracheal glands.
Neuropeptides degranulate serous cells of ferret tracheal glands.
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神经肽使雪貂气管腺的浆液细胞脱粒。
DOI：
10.1152/ajpcell.1986.251.2.c223
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Basbaum,CB
中科院分区：
文献类型：
--
作者：
Gashi,AA;Borson,DB;Finkbeiner,WE;Nadel,JA;Basbaum,CB
为了确定是否在气管粘膜下腺体的浆液或粘液细胞响应的神经肽P物质（SP）和血管活性肠肽（VIP），我们研究了肽诱导的腺细胞形态的变化，伴随35 SO 4标记的大分子从气管外植体的雪貂释放。将外植体在含有35 SO 4的培养基中标记1小时，并再洗涤3.5小时。基线分泌在没有药物的情况下下降1.5和3.5小时后脉冲。在2.5和3.5 h之间，每个采样周期每分钟回收的计数的平均百分比变化与零无显著差异（P> 0.3; n = 6）。标记后4 h分别加入P物质（10 ~（-5）M）和VIP（2 × 10 ~（-6）M），使~（35）SO_4标记大分子的释放量（SP，219%; VIP，180%）明显高于基线。氨甲酰胆碱是一种毒蕈碱胆碱能激动剂（10（-5）M），使分泌量平均增加142%，高于基线（每种效应，P <0.05;每种n = 6）。对照组织的光镜和电镜显示腺体管腔狭窄，分泌颗粒众多。用SP或VIP处理的腺体管腔扩大，浆液细胞明显脱颗粒。这些现象被记录的形态计量学和表明，SP和VIP引起腺体分泌至少部分通过刺激分泌从浆液细胞。
To determine whether serous or mucous cells in tracheal submucosal glands respond to the neuropeptides substance P (SP) and vasoactive intestinal peptide (VIP), we studied the peptide-induced changes in gland cell morphology accompanying release of 35SO4-labeled macromolecules from tracheal explants of ferrets. Explants were labeled for 1 h in medium containing 35SO4 and washed for 3.5 additional hours. Base-line secretion in the absence of drugs declined between 1.5 and 3.5 h after the pulse. Between 2.5 and 3.5 h, the average percent change in counts per minute recovered per sample period was not significantly different from zero (P greater than 0.3; n = 6). Substance P (10(-5) M) and VIP (2 X 10(-6) M) added 4 h after labeling each increased greatly the release of 35SO4-labeled macromolecules (SP, 219%; VIP, 180%) above base line. Bethanechol, a muscarinic-cholinergic agonist (10(-5) M), increased secretion by an average of 142% above base line (each effect, P less than 0.05; n = 6 each). Light and electron microscopy of the control tissues showed glands with narrow lumens and numerous secretory granules. Glands treated with SP or VIP had enlarged lumens and the serous cells were markedly degranulated. These phenomena were documented by morphometry and suggest that SP and VIP cause secretion from glands at least partially by stimulating exocytosis from serous cells.