Role of MAML1 and MEIS1 in Esophageal Squamous Cell Carcinoma Depth of Invasion
Role of MAML1 and MEIS1 in Esophageal Squamous Cell Carcinoma Depth of Invasion
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DOI：
10.1007/s12253-017-0243-1
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发表时间：
2018-04-01
影响因子：
2.8
通讯作者：
Moghbeli, Meysam
中科院分区：
文献类型：
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作者：
Abbaszadegan, Mohammad Reza;Moghbeli, Meysam
同源盒（HOX）转录因子和NOTCH信号通路是胃肠道组织发育过程中干细胞功能、细胞命运和稳态的重要调节因子。在本研究中，我们通过评估食管鳞状细胞癌（ESCC）患者中分别作为NOTCH通路主要转录因子和HOX转录机制增强子的maml1和MEIS1之间可能的相关性，分析了NOTCH通路和HOX基因之间的串扰。采用实时聚合酶链反应（real-time polymerase chain reaction， PCR）评估51例ESCC患者Meis1和Maml1 mRNA的表达水平。51例患者中仅有3例（5.9%）MEIS1/MAML1低表达，2/51 (3.9%)MEIS1/MAML1过表达。51个样本中有9个（17.6%）显示MEIS1低表达和MAML1过表达。maml1与MEIS1mRNA表达有显著相关性（p < 0.05）。MEIS1过表达与肿瘤部位（p = 0.05）、肿瘤浸润深度（p = 0.011）、性别（p = 0.04）有显著相关性。我们的研究结果表明，在ESCC的进展过程中，MEIS1可能对maml1的表达有负调控作用。
Homeobox (HOX) transcription factors and NOTCH signaling pathway are critical regulators of stem cell functions, cell fate in development and homeostasis of gastrointestinal tissues. In the present study, we analyzed cross talk between NOTCH pathway and HOX genes through assessment of probable correlation betweenMAML1 and MEIS1 as the main transcription factor of NOTCH pathway and enhancer of HOX transcriptional machinery, respectively in esophageal squamous cell carcinoma (ESCC) patients. Fifty one ESCC cases were enrolled to assess the levels of Meis1 and Maml1 mRNA expression using real-time polymerase chain reaction (PCR). Only 3 out of 51 (5.9%) cases had MEIS1/MAML1 under expression and 2/51 (3.9%) cases had MEIS1/MAML1over expression. Nine out of 51 samples (17.6%) have shown MEIS1 under expression and MAML1 over expression. There was a significant correlation between MAML1and MEIS1mRNA expressions (p 0.05). There were significant correlations between MEIS1 under/MAML1 over expressed cases and tumor location (p = 0.05), tumor depth of invasion (p = 0.011), and sex (p = 0.04). Our results showed that MEIS1 may have a negative role in regulation of MAML1expression during the ESCC progression.