The impact of nucleosome positioning on the organization of replication origins in eukaryotes.
The impact of nucleosome positioning on the organization of replication origins in eukaryotes.
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DOI：
10.1016/j.bbrc.2009.05.072
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发表时间：
2009-07
影响因子：
3.1
通讯作者：
Shanye Yin;Wenjun Deng;Landian Hu;Xiangyin Kong
中科院分区：
文献类型：
--
作者：
Shanye Yin;Wenjun Deng;Landian Hu;Xiangyin Kong
真核生物染色体上DNA复制起点（ORI）的分布是非随机的，但其背后的原因还不清楚。以前的研究表明，在决定ORI组织的转录活性的突出作用。在这里，我们确定核小体占用作为一个可能的候选人建立ORI分布。结合酵母和人类核小体定位和ORI组织的全基因组数据，我们证明了开放的染色质结构域，其特征在于核小体耗尽，优先允许复制。然而，与先验的说法相反，转录活性的影响比以前提出的要弱得多，并且可以部分地用我们的核小体排除模型来解释。我们认为，核小体定位所施加的ORI组织在真核生物中广泛存在。
The distribution of DNA replication origins (ORIs) on eukaryotic chromosomes is nonrandom, but the reasons behind this are not well understood. Previous studies have suggested a prominent role of transcriptional activity in determining the ORI organization. Here, we identify nucleosome occupancy as a likely candidate to set up ORI distribution. Combining genome-wide data on nucleosome positioning and ORI organization in yeast and humans, we demonstrate that open chromatin domains, characterized by nucleosome depletion, are preferentially permissive for replication. However, contrary to priori claims, the impact of transcriptional activity is considerably weaker than previously proposed and could partly be explained by our nucleosome exclusion model. We propose that the ORI organization imposed by nucleosome positioning is phylogenetically widespread in eukaryotes.