PURIFIED HEPATOCYTE NUCLEAR FACTOR-I INTERACTS WITH A FAMILY OF HEPATOCYTE-SPECIFIC PROMOTERS
PURIFIED HEPATOCYTE NUCLEAR FACTOR-I INTERACTS WITH A FAMILY OF HEPATOCYTE-SPECIFIC PROMOTERS
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DOI：
10.1073/pnas.85.21.7937
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
CRABTREE, GR
中科院分区：
文献类型：
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作者：
COURTOIS, G;BAUMHUETER, S;CRABTREE, GR
在发育过程中，细胞类型是通过特定基因类别的激活或抑制而产生的。一种假设是，这种现象是由在转录水平发挥作用的组织特异性因子实现的。最近我们描述了一种肝脏特异性核蛋白，肝细胞核因子1，它似乎参与纤维蛋白原和α1-抗胰蛋白酶基因的转录。在本报告中，我们描述了肝细胞核因子 1 的纯化，并证明它与许多肝脏特异性基因的必需启动子区域相互作用，包括白蛋白、甲胎蛋白和运甲状腺素蛋白。这一发现表明肝细胞核因子 1 可能是建立肝脏表型所必需的因素之一。我们还表明，这种蛋白质与乙型肝炎病毒表面抗原基因的启动子结合，乙型肝炎病毒是一种具有高度嗜肝性的病毒。
During development cell types arise through the activation or repression of classes of specific genes. One hypothesis is that this phenomenon is realized by tissue-specific factors playing a role at the transcription level. Recently we have described a liver-specific nuclear protein, hepatocyte nuclear factor 1, that appears to be involved in the transcription of the fibrinogen and .alpha.1-antitrypsin genes. In this report we described the purfication of hepatocyte nuclear factor 1 and demonstrate that it interacts with essential promoter regions of many liver-specific genes, including albumin, .alpha.-fetoprotein and transthyretin. This finding suggests that hepatocyte nuclear factor 1 could be one factor necessary for establishing the liver phenotype. We also show that this protein binds to the promoter of the surface-antigen gene of the hepatitis B virus, a virus characterized by a high degree of hepatotropism.