Integrated bioprocess for bio-ethanol production from watermelon rind biomass: Ultrasound-assisted deep eutectic solvent pretreatment, enzymatic hydrolysis and fermentation
Integrated bioprocess for bio-ethanol production from watermelon rind biomass: Ultrasound-assisted deep eutectic solvent pretreatment, enzymatic hydrolysis and fermentation
复制标题
DOI：
10.1016/j.renene.2021.08.057
复制
发表时间：
2021-08
期刊：
影响因子：
8.7
通讯作者：
O. A. Fakayode;N. Akpabli-Tsigbe;Hafida Wahia;Shanshan Tu;Manni Ren;Cunshan Zhou;Haile Ma
中科院分区：
文献类型：
--
作者：
O. A. Fakayode;N. Akpabli-Tsigbe;Hafida Wahia;Shanshan Tu;Manni Ren;Cunshan Zhou;Haile Ma
以西瓜皮为原料，采用超声波和低共熔溶剂（DES）联合预处理、酶解和发酵相结合的工艺，研究了西瓜皮生物质生产生物乙醇的潜力。为了提高WMR转化为生物乙醇的效率，采用中心组合旋转试验设计，研究了超声辅助DES预处理对生物乙醇产率的影响。葡萄糖是WMR生物质中最主要的糖，这增强了其作为发酵过程的廉价碳源的适合性。最大葡萄糖和总还原糖得率分别为60.17%和83.03%，酶解效率为75.79%~ 91.13%。在超声功率180 W、超声频率40 k H z、超声时间40 min、DES反应温度120° C和DES反应时间180 min的条件下，生物乙醇产量范围为0.276至0.458 g g-1粗WMR，对应于54.12- 89.80%的发酵效率。结果表明，所考虑的预处理因素对生物乙醇产率均有显著影响。结果表明，预处理能有效地脱除纤维素纤维中的半纤维素，使纤维素和半纤维素脱木素、增溶并水解为单糖。这提高了可发酵糖的回收率，从而提高了生物乙醇的产量。
Herein, the potential of watermelon rind (WMR) biomass for bio-ethanol production through an integrated bioprocess consisting of combinative sequential ultrasonication and deep eutectic solvent (DES) pretreatments, enzymatic hydrolysis and fermentation was investigated. For the improvement of the efficiency of the WMR conversion into bio-ethanol, the effectiveness of the ultrasonic-assisted DES pretreatment on bio-ethanol yield was studied by optimization, adopting Central Composite Rotatable Design. Glucose was the most dominant sugar in the WMR biomass which enhanced its suitability as cheap carbon source for fermentation process. Maximum glucose and total reducing sugar yields of 60.17% and 83.03% were respectively obtained, and the enzymatic hydrolysis efficiencies ranged from 75.79 to 91.13%. The bio-ethanol yield ranged from 0.276 to 0.458 g g− 1 raw WMR, corresponding to fermentation efficiencies of 54.12–89.80%, with the maximum obtained at ultrasonication power 180 W, ultrasonication frequency 40 k H z, ultrasonication time 40 m i n, DES reaction temperature 120° C and DES reaction time 180 m i n. Results showed that all the pretreatment factors considered had significant effect on bio-ethanol yield. It was established that combinative pretreatment was efficient for delignification, solubilization and hydrolysis of cellulose and hemicellulose into monomeric sugars by removing hemicellulose from the cellulose fibers. This enhanced fermentable sugar recovery, which consequently increased bio-ethanol yield.