A new continuous alternation task in T-maze detects hippocampal dysfunction in mice - A strain comparison and lesion study
A new continuous alternation task in T-maze detects hippocampal dysfunction in mice - A strain comparison and lesion study
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DOI：
10.1016/s0166-4328(97)00214-3
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发表时间：
1998-09-01
影响因子：
2.7
通讯作者：
Gerlai, R
中科院分区：
文献类型：
--
作者：
Gerlai, R
哺乳动物海马体因其重要的行为功能以及长时程增强（LTP）是该大脑区域的显着特征而成为多项神经生物学研究的焦点。越来越多的证据表明，海马功能与学习环境线索的多种关系有关。本文介绍了一种新颖的行为测试程序，即改进的 T 迷宫连续交替任务（T-CAT），除了经常应用的水迷宫和恐惧条件反射范式之外，它还可以作为海马功能的简单、可自动化和快速的测试。对海马受到鹅膏菌酸损伤或注射媒介物的小鼠（品系 C57BL/6）、过表达钙结合蛋白 S100 β 的两种转基因品系（CD1 背景）以及近交系（C57BL/6、DBA/2、129/SV 和 129/SVEV）和远交系（CD1）小鼠品系进行比较。这项研究表明海马损伤导致 T-CAT 显着受损。此外，S100 beta 的过度表达会损害海马 LTP，也会导致损害，表明 T-CAT 对检测海马功能障碍很敏感。对小鼠品系的分析显示，C57BL/6 和 CD1 小鼠在 T-CAT 中表现良好，而 129/SV、129/SVEV 和 DBA/2 则显着受损，这一发现强调了品系差异在小鼠海马功能的药理学或单基因操作研究中的重要性。 (C) 1998 Elsevier Science B.V. 保留所有权利。
The mammalian hippocampus has been the focus of several neurobiology studies because of its important behavioral function and because long-term potentiation (LTP) is a prominent feature of this brain region. Converging evidence suggests that hippocampal function is associated with learning multiple relationships of environmental cues. In this paper a novel behavioral test procedure is introduced, a modified T-maze continuous alternation task (T-CAT), that may serve as a simple, automatable, and quick test of hippocampal function in addition to the frequently applied water maze and fear conditioning paradigms. A comparison is made between mice (strain C57BL/6) with ibotenic acid lesioned or vehicle injected hippocampus, two transgenic strains (on CD1 background) overexpressing a calcium binding protein, S100 beta, and inbred (C57BL/6, DBA/2, 129/SV and 129/SVEV) and outbred (CD1) strains of mice. This study shows that hippocampal lesioning led to a significant impairment in T-CAT. Furthermore, overexpression of S100 beta, which impairs hippocampal LTP, also led to an impairment demonstrating that T-CAT is sensitive to detect hippocampal dysfunction. Analysis of the mouse strains revealed that C57BL/6 and CD1 mice performed well in T-CAT, whereas 129/SV, 129/SVEV and DBA/2 were significantly impaired, a finding that underscores the importance of strain differences in pharmacological or single gene manipulation studies of hippocampal function in mice. (C) 1998 Elsevier Science B.V. All rights reserved.