Recent insight into class-specific properties of murine immunoglobulins obtained with the help of monoclonal antibodies.
Recent insight into class-specific properties of murine immunoglobulins obtained with the help of monoclonal antibodies.
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最近通过单克隆抗体获得的鼠免疫球蛋白的类别特异性特性的见解。
DOI：
10.1159/000233205
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Ovary,Z
中科院分区：
文献类型：
--
作者：
Ovary,Z
BALB/c and C57BL/6 mice were immunized with monoclonal anti-DNP IgE, obtained by fusion of PX63Ag8–6-5–3 cells with spleen cells from immunized C57BL/6 or BALB/c mice, respectively. With the antiallotype sera thus produced the allotype of IgE (i.e. 7) could be defined since BALB/c is of the Igh-a and C57BL/6 of the Igh-b allotype. The antiallotype 7b does not cross-react with other allotypes. Antiallotype 7a recognizes in addition to allotype 7a also allotypes 7j, 7d, and 7e as expected. Both the complement fixing and the sensitizing activities of murine anti-TNP and anti-DNP monoclonal antibodies were examined. The minimal amount/ml for complement fixation was 0.46μg from one of the IgGl antibodies, 0.46 from IgG2a, 12.5 ng from IgM, 0.7μg from IgG3. Complement fixation by IgGl was unexpected. The minimal amount/ml for mouse PC A was 0.5–0.75μg from IgGl, 1.44–2.3μg from IgG2a, 5.6μg from purified IgG2b and about 15 ng from IgE. Sensitization by IgG2a and IgG2b were new findings. Minimal amount/ml for guinea pig PCA was 0.23μg from IgG2a. Minimal amount/ml for rat PCA was 2 ng from IgE.
