Deduced amino acid sequence of bovine retinal Go alpha: similarities to other guanine nucleotide-binding proteins.
Deduced amino acid sequence of bovine retinal Go alpha: similarities to other guanine nucleotide-binding proteins.
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牛视网膜 Go α 的推导氨基酸序列：与其他鸟嘌呤核苷酸结合蛋白的相似性。
DOI：
10.1073/pnas.84.10.3107
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发表时间：
1987
影响因子：
11.1
通讯作者：
Vaughan,M
中科院分区：
文献类型：
--
作者：
VanMeurs,KP;Angus,CW;Lavu,S;Kung,HF;Czarnecki,SK;Moss,J;Vaughan,M
通过使用与牛转导蛋白 (T α) α 亚基的两个推定克隆中已公布序列互补的寡核苷酸探针，分离出编码 Go α（一种鸟嘌呤核苷酸结合蛋白（G 蛋白））完整序列（354 个氨基酸）的牛视网膜 cDNA 克隆。推导的氨基酸序列包含与牛脑 Go α 的 7 个胰蛋白酶肽（总共 63 个氨基酸）相同的序列。牛视网膜 Go α 的 cDNA 在编码区和 3' 非翻译区中与最近描述的来自大鼠脑的 Go α 部分 cDNA 克隆具有大于 90% 的同一性卡齐罗，Y. (1986) Proc。国家。阿卡德。科学。美国 83，3776-3780]。将牛 Go α 克隆的核苷酸和推导的氨基酸序列与先前报道的其他牛源 G 蛋白（Gs α、Gi α 和 T α）的核苷酸序列和推导的氨基酸序列进行比较，揭示了与霍乱毒素和百日咳毒素修饰的氨基酸周围的区域相同的广泛区域。 G 蛋白、延伸因子和 ras p21 基因产物的区域也存在明显的序列相似性，据信这些区域参与鸟嘌呤核苷酸结合和 GTP 水解。
A bovine retinal cDNA clone encoding the complete sequence (354 amino acids) of Go alpha, a guanine nucleotide-binding protein (G protein), was isolated by using oligonucleotide probes complementary to published sequences in two putative clones for the alpha subunit of bovine transducin (T alpha). The deduced amino acid sequence contained sequences identical to those in seven tryptic peptides (total 63 amino acids) from bovine brain Go alpha. The cDNA for bovine retinal Go alpha exhibits greater than 90% identity in both coding and 3' untranslated regions with a recently described partial cDNA clone for Go alpha from rat brain [Itoh, H., Kozasa, T., Nagata, S., Nakamura, S., Katada, T., Ui, M., Iwai, S., Ohtsuka, E., Kawasaki, H., Suzuki, K. & Kaziro, Y. (1986) Proc. Natl. Acad. Sci. USA 83, 3776-3780]. Comparison of the nucleotide and deduced amino acid sequences of the bovine Go alpha clone with those previously reported for other G proteins of bovine origin (Gs alpha, Gi alpha, and T alpha) reveals extensive regions identical to those surrounding the amino acids modified by cholera toxin and pertussis toxin. There are also marked similarities of sequence in regions of the G proteins, elongation factors, and the ras p21 gene products that are believed to be involved in guanine nucleotide binding and GTP hydrolysis.