Systems Pharmacology-based strategy to screen new adjuvant for hepatitis B vaccine from Traditional Chinese Medicine Ophiocordyceps sinensis.
Systems Pharmacology-based strategy to screen new adjuvant for hepatitis B vaccine from Traditional Chinese Medicine Ophiocordyceps sinensis.
复制标题
基于系统药理学策略从中药冬虫夏草中筛选乙型肝炎疫苗新佐剂
DOI：
10.1038/srep44788
复制
发表时间：
2017-03-20
影响因子：
4.6
通讯作者：
Zhu N
中科院分区：
文献类型：
--
作者：
Wang J;Liu R;Liu B;Yang Y;Xie J;Zhu N
佐剂是许多疫苗的常见成分，但由于效率低或副作用，仍很少有许可用于人类使用。本研究采用系统药理学分析方法，从中药中筛选佐剂。蛇珊瑚在中国和其他亚洲国家已使用多年，具有许多生物学特性，但药理机制尚未完全阐明。首先，在这项研究中，190个推定的目标，17个活性化合物的Ophiocorphisinensis检索和系统药理学为基础的方法被应用到提供新的见解药物的药理作用。途径富集分析发现，靶点参与了几个免疫过程。基于此，我们选择虫草素作为目标化合物作为B型肝炎疫苗的佐剂，因为现有的疫苗往往不能在许多受试者中诱导有效的免疫应答。动物和细胞实验最终验证了新疫苗同时提高了BALB/c小鼠的体液免疫和细胞免疫，且无副作用。这些结果表明，虫草素可以作为B肝炎疫苗的佐剂，系统药理学分析可以作为佐剂选择的新方法。
Adjuvants are common component for many vaccines but there are still few licensed for human use due to low efficiency or side effects. The present work adopted Systems Pharmacology analysis as a new strategy to screen adjuvants from traditional Chinese medicine. Ophiocordyceps sinensis has been used for many years in China and other Asian countries with many biological properties, but the pharmacological mechanism has not been fully elucidated. First in this study, 190 putative targets for 17 active compounds in Ophiocordyceps sinensis were retrieved and a systems pharmacology-based approach was applied to provide new insights into the pharmacological actions of the drug. Pathway enrichment analysis found that the targets participated in several immunological processes. Based on this, we selected cordycepin as a target compound to serve as an adjuvant of the hepatitis B vaccine because the existing vaccine often fails to induce an effective immune response in many subjects. Animal and cellular experiments finally validated that the new vaccine simultaneously improves the humoral and cellular immunity of BALB/c mice without side effects. All this results demonstrate that cordycepin could work as adjuvant to hepatitis b vaccine and systems-pharmacology analysis could be used as a new method to select adjuvants.