Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray
Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray
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DOI：
10.1046/j.1365-313x.2002.01359.x
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发表时间：
2002-08-01
期刊：
影响因子：
7.2
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Seki, M;Narusaka, M;Shinozaki, K
全长cDNA对于拟南芥基因组测序后的植物基因功能分析是必不可少的。最近，cDNA微阵列分析已被开发用于表达谱的全局和同时分析的定量分析。我们制备了包含约7000个独立全长cDNA组的全长cDNA微阵列，以分析干旱、寒冷（低温）和高盐胁迫条件下基因随时间的表达谱。在低温、干旱和高盐胁迫下，分别有53、277和194个基因的转录量增加，与对照相比增加了5倍以上。我们还发现了许多高度干旱，寒冷或高盐胁迫诱导基因。然而，我们观察到强烈的关系，这些压力响应基因的表达的基础上维恩图分析，并发现22个压力诱导基因，响应所有三个压力。几个基因组显示不同的表达谱进行了鉴定，通过分析他们的表达模式在胁迫响应基因诱导。根据冷诱导基因的表达谱，将其分为至少两个基因组。DREB 1A包括在冷处理后2 h表达达到峰值的组中。在已鉴定的干旱、低温或高盐胁迫诱导基因中，共发现40个转录因子基因（约占已鉴定胁迫诱导基因的11%），表明干旱、低温或高盐胁迫信号转导途径中存在多种转录调控机制。
Full-length cDNAs are essential for functional analysis of plant genes in the post-sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full-length cDNA microarray containing approximate to7000 independent, full-length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high-salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought-, cold- or high-salinity- stress-inducible genes. However, we observed strong relationships in the expression of these stress-responsive genes based on Venn diagram analysis, and found 22 stress-inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress-responsive gene induction. The cold-inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high-salinity stress-inducible genes identified, we found 40 transcription factor genes (corresponding to approximate to11% of all stress-inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high-salinity stress signal transduction pathways.