Altered starch structure is associated with endosperm modification in Quality Protein Maize
Altered starch structure is associated with endosperm modification in Quality Protein Maize
复制标题
DOI：
10.1073/pnas.2136854100
复制
发表时间：
2003-12-23
影响因子：
11.1
通讯作者：
Larkins, BA
中科院分区：
文献类型：
--
作者：
Gibbon, BC;Wang, XL;Larkins, BA
人们对优质蛋白玉米（QPM）籽粒质地改良的生化基础知之甚少。几个QPM系的蛋白质组学分析表明，这些基因型的可溶性非玉米醇溶蛋白组分中颗粒结合淀粉合成酶I的水平增加。这种酶的提取增加反映了淀粉结构的变化，这是表现为较短的支链淀粉分支和增加淀粉颗粒溶胀。在成熟的籽粒中，这些淀粉结构的改变与淀粉颗粒之间的相互联系有关，导致玻璃状籽粒表型。了解这种以前未表征的淀粉结构的分子基础将加速QPM的发展。
The biochemical basis of modified kernel texture in Quality Protein Maize (QPM) is poorly understood. Proteomic analysis of several QPM lines indicated increased levels of granule-bound starch synthase I in the soluble nonzein protein fraction of these genotypes. Increased extraction of this enzyme reflected a change in starch structure, which was manifested as shorter amylopectin branches and increased starch-granule swelling. In mature kernels, these alterations in starch structure were associated with interconnections between starch granules that resulted in a vitreous kernel phenotype. Understanding the molecular basis for this previously uncharacterized starch structure will accelerate the development of QPM.