PRODUCTION OF SOLVENTS BY CLOSTRIDIUM-ACETOBUTYLICUM CULTURES MAINTAINED AT NEUTRAL PH
PRODUCTION OF SOLVENTS BY CLOSTRIDIUM-ACETOBUTYLICUM CULTURES MAINTAINED AT NEUTRAL PH
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DOI：
10.1128/aem.48.6.1166-1170.1984
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发表时间：
1984-01-01
影响因子：
4.4
通讯作者：
MORRIS, JG
中科院分区：
文献类型：
--
作者：
HOLT, RA;STEPHENS, GM;MORRIS, JG
在35℃的间歇培养条件下，对C. acetobutylicum NCIB 8052 （ATCC 824）生成丙酮和正丁醇的情况进行了监测。C在葡萄糖（2% [wt/vol]）最小培养基中保持在pH 5.0或7.0。在pH为5时，在未添加的培养基中获得了良好的溶剂生产，尽管添加乙酸和丁酸盐（各10 mM）导致在较低的生物质浓度下开始溶剂生产。在pH为7时，虽然在未添加的培养基中保持了纯粹的产酸发酵，但当培养基中的葡萄糖含量增加（至4% [wt/vol]）时，产生了低浓度的丙酮和正丁醇。在pH为7的2%葡萄糖培养基中，添加高浓度的乙酸和丁酸盐（各100 mM，作为K盐供应），获得大量的溶剂浓度。C. acetobutylicum NCIB 8052与C. beijerinckii VPI 13436一样，能够在中性pH下产生溶剂，尽管只有在提供足够高浓度的乙酸和丁酸盐时才能获得良好的收率。在pH为5的葡萄糖培养基中添加丙酸（20 mM），可以产生一些正丙醇、丙酮和正丁醇；最终培养基几乎不含酸。在pH为7时，添加丙酸（150 mM）再次导致了正丙醇的形成，也引起了一些丙酮和正丁醇的产生，尽管在相同的基础培养基中添加乙酸和丁酸在pH为7时所获得的数量要少得多。
The formation of acetone and n-butanol by C. acetobutylicum NCIB 8052 (ATCC 824) was monitored in batch culture at 35.degree. C in a glucose (2% [wt/vol]) minimal medium maintained throughout at pH 5.0 or 7.0. At pH 5, good solvent production was obtained in the unsupplemented medium, although addition of acetate plus butyrate (10 mM each) caused solvent production to be initiated at a lower biomass concentration. At pH 7, although a purely acidogenic fermentation was maintained in the unsupplemented medium, low concentrations of acetone and n-butanol were produced when the glucose content of the medium was increased (to 4% [wt/vol]). Substantial solvent concentrations were obtained at pH 7 in the 2% glucose medium supplemented with high concentrations of acetate plus butyrate (100 mM each, supplied as their K salts). C. acetobutylicum NCIB 8052, like C. beijerinckii VPI 13436, is able to produce solvents at neutral pH, although good yields are obtained only when adequately high concentrations of acetate and butyrate are supplied. Supplementation of the glucose minimal medium with propionate (20 mM) at pH 5 led to the production of some n-propanol and acetone and n-butanol; the final culture medium was virtually acid free. At pH 7, supplementation with propionate (150 mM) again led to the formation of n-propanol and also provoked production of some acetone and n-butanol, although in considerably smaller amounts than were obtained when the same basal medium was fortified with acetate and butyrate at pH 7.