Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection
Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection
复制标题
华支睾吸虫共感染调节小鼠宿主对旋毛虫感染的反应
DOI：
10.1007/s00436-013-3493-1
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发表时间：
2013-09-01
影响因子：
2
通讯作者：
Lu, Fangli
中科院分区：
文献类型：
--
作者：
Chen, Ying;Huang, Bo;Lu, Fangli
不同种类寄生虫的伴随感染在该领域很常见。感染一种寄生虫可能引发宿主反应，这种反应可能影响另一种寄生虫的后续感染并改变疾病结果。到目前为止，大多数研究都集中在单一种类寄生虫感染上，首次寄生虫感染对二次感染所诱导的保护机制仍不明确。在这项研究中，我们评估了华支睾吸虫感染对另一种组织线虫旋毛虫感染过程的影响。我们观察到，预先感染华支睾吸虫的小鼠肠道内旋毛虫的虫体负荷比单独感染旋毛虫的小鼠低；预先感染华支睾吸虫的小鼠在受到旋毛虫攻击时，肠道组织病理学变化也更严重，肠道潘氏细胞数量更多。采用实时定量聚合酶链反应分析了不同组小鼠小肠和脾脏中白细胞介素（IL）-4、IL - 10、IL - 13和肿瘤坏死因子（TNF）-α的mRNA水平。与单独感染旋毛虫的小鼠相比，双重感染小鼠的小肠组织中IL - 13的mRNA表达显著增加，脾脏组织中IL - 4、IL - 13和TNF -α显著增加。我们的研究结果表明，华支睾吸虫的“预先存在”的吸虫感染是一个有助于减少旋毛虫成虫在小肠内寄生的因素。
Concomitant infections of different species of parasites are common in the field. Infection with one parasite species likely triggers host responses that may influence the subsequent infection of another species and alter disease outcomes. So far, the majority of studies have focused on single species parasite infection, and the mechanisms of protection induced by the first parasite infection against the secondary infection remain poorly defined. In this study, we assess the impact of trematode Clonorchis sinensis infection on the course of another tissue nematode Trichinella spiralis challenge. We observed that mice with preexisting C. sinensis infection had lower worm burden of intestinal T. spiralis than those infected with T. spiralis alone; mice with preexisting C. sinensis also had severe enteric histopathological changes and higher counts of intestinal Paneth cells in responses to T. spiralis challenge. The mRNA levels of interleukin (IL)-4, IL-10, IL-13, and tumor necrosis factor (TNF)-α from the small intestine and spleen of the different groups were analyzed using quantitative real-time polymerase chain reaction. Compared with that in mice infected with T. spiralis alone, the mRNA expression of IL-13 was significantly increased in the small intestine tissues and IL-4, IL-13, and TNF-α were significantly increased in the spleen tissues in the dually infected mice. Our findings suggest that a "preexisting" trematode infection of C. sinensis is a factor which contributes to reducing the establishment of T. spiralis adult worms in the small intestine.