Time-course proteomic profile of Candida albicans during adaptation to a fetal serum
Time-course proteomic profile of Candida albicans during adaptation to a fetal serum
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适应胎儿血清过程中白色念珠菌的时程蛋白质组图谱
DOI：
10.1111/2049-632x.12003
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发表时间：
2013-02-01
影响因子：
3.3
通讯作者：
Ueda, Mitsuyoshi
中科院分区：
文献类型：
--
作者：
Aoki, Wataru;Ueda, Tomomi;Ueda, Mitsuyoshi
白色念珠菌是一种共生微生物，但如果宿主免疫受损，它会导致致命的疾病。由于缺乏有效的治疗，死亡率非常高，导致对新药的需求源源不断。本研究描述了白念珠菌对胎牛血清(FBS)适应过程中的蛋白质组学。时间进程蛋白质组学是理解在动态变化的环境中进行适应的确切过程的一种有前途的方法。白念珠菌在酵母氮基(YNB)+/-FBS培养基中培养，用LC-MS/MS系统和长整体硅胶毛细管柱鉴定了孵育0或60min的终点样品中的1418个蛋白质。接下来，我们对YNB+FBS样本进行了时间进程蛋白质组学研究，以确定适应FBS的首要蛋白质。我们鉴定了16个蛋白为新生蛋白/新合成的蛋白，它们被认为是重要的毒力因子候选。基因本体论分析表明，与运输相关的蛋白在这16种蛋白中含量较高，这表明白念珠菌可能在时间上优先获得必需元素。白念珠菌的时间进程蛋白质组学揭示了适应FBS的优先顺序。刻画时程动态将有助于深刻理解白念珠菌的毒力。
Candida albicans is a commensal organism; however, it causes fatal diseases if the host immunity is compromised. The mortality rate is very high due to the lack of effective treatment, leading to ceaseless demand for novel pharmaceuticals. In this study, time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) was described. Time-course proteomics is a promising way to understand the exact process of going adaptation in dynamically changing environments. Candida albicans was cultivated in yeast nitrogen base (YNB) +/- FBS media, and we identified 1418 proteins in the endpoint samples incubated for 0 or 60 min by a LC-MS/MS system with a long monolithic silica capillary column. Next, we carried out time-course proteomics of the YNB + FBS samples to identify top-priority proteins for adaption to FBS. We identified 16 proteins as nascent/newly synthesized proteins, and they were recognized as candidates of important virulent factors. Gene ontology analysis revealed that transport-related proteins were enriched in the 16 proteins, indicating that C. albicans probably put priority in time on the acquisition of essential elements. Time-course proteomics of C. albicans revealed the order of priority to adapt to FBS. Depicting time-course dynamics will lead to profound understandings of virulence of C. albicans.