Advances in CE for kinetic studies
Advances in CE for kinetic studies
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动力学研究中 CE 的进展
DOI：
10.1002/elps.200700086
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发表时间：
2008
期刊：
影响因子：
2.9
通讯作者：
G. Collins
中科院分区：
文献类型：
--
作者：
Carl I D Newman;G. Collins
CE是一种很有前途的研究分子相互作用的技术，因为它提供了多种相互作用模式的评估，不需要固定分子，没有死时间。为了进行这些研究，已经开发了许多方法来确定结合常数和其他热力学参数。近年来对这些方法进行了广泛的综述。然而，测定反应速率的方法较少。尽管如此，近年来已经取得了大量的理论和实验进展来解决这一差异。其中一些方法采用计算机模拟来确定一阶或二阶速率常数，而其他方法直接计算速率常数作为解析方程的解。这篇综述的目的是对这些方法的基本假设、实验方法、速率常数的计算和固有的局限性进行描述。
CE is a promising technique for the investigation of molecular interactions because it affords evaluation of multiple interaction modes, does not require immobilization of molecules, and has no dead time. In order to perform these investigations, numerous methods have been developed for determining binding constants and other thermodynamic parameters. These methods have been reviewed extensively in recent years. However, methods for determining the rates of reaction are less prolific. Nonetheless, numerous theoretical and experimental advances have been made in recent years to address this discrepancy. Some of these methods employ computer simulations to determine first‐order or second‐order rate constants numerically, whereas other methods calculate rate constants directly as solutions to analytical equations. It is the object of this review to provide descriptions of these methods in terms of their underlying assumptions, experimental methodology, calculation of rate constants, and inherent limitations.
肽基脯氨酸二肽顺反异构化动力学研究。
DOI：
10.1016/0021-9673(96)00241-5
发表时间：
1996
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Thunecke,F;Kálmán,A;Kálmán,F;Ma,S;Rathore,AS;Horváth,C
通讯作者：
Horváth,C
使用亲和毛细管电泳测定含有 N-2,4-二硝基苯基的配体与二价单克隆大鼠抗 DNP 抗体的结合。
影响因子：
7.4
作者：
Mammen,M;Gomez,FA;Whitesides,GM
通讯作者：
Whitesides,GM