CYTOKERATINS AS EPITHELIAL DIFFERENTIATION MARKERS IN PREMALIGNANT AND MALIGNANT ORAL LESIONS
CYTOKERATINS AS EPITHELIAL DIFFERENTIATION MARKERS IN PREMALIGNANT AND MALIGNANT ORAL LESIONS
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DOI：
10.1111/j.1600-0714.1992.tb00960.x
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发表时间：
1992-01-01
影响因子：
3.3
通讯作者：
BRANDTZAEG, P
中科院分区：
文献类型：
--
作者：
HEYDEN, A;HUITFELDT, HS;BRANDTZAEG, P
口腔复层上皮角蛋白的表达模式与细胞分化程度有关。正常模式显示基底层中的角蛋白对K5和K14，而K1和K10或K4和K13是与分化的基底上细胞相关的两对。用单克隆和多克隆抗体对单个角蛋白（K10，K13和K14）进行双色免疫荧光染色，以研究它们在单个细胞中的共表达。癌前病变和恶性病变中角蛋白表达的改变提示分化异常。单特异性角蛋白抗体可用于评价口腔粘膜上皮分化异常和口腔鳞状细胞癌的分化程度。
The keratin expression pattern in oral stratified epithelium is related to the cellular differentiation level. The normal pattern shows the keratin pair K5 and K14 in the stratum basale whereas K1 and K10, or K4 and K13, are the two pairs associated with differentiating suprabasal cells. Monoclonal and polyclonal antibodies to individual keratins (K10, K13 and K14) were used in a two-color immunofluorescence staining method to study their coexpression in single cells. Altered keratin expression in premalignant and malignant lesions indicated abnormal differentiation. Monospecific keratin antibodies were suggested to be useful for evaluation of epithelial differentiation changes in oral dysplasias and oral squamous cell carcinomas.