Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo
Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo
复制标题
桐城猪与体内人工感染PRRSV的大白猪周围免疫器官选择性剪接差异的整体分析
DOI：
10.1155/2020/4045204
复制
发表时间：
2020-01
影响因子：
--
通讯作者：
Liu Bang
中科院分区：
文献类型：
--
作者：
Zhang Yu;Xue Liyao;Xu Hang;Liang Wan;Wu Qingqing;Zhang Qingde;Zhou Xiang;Liu Bang
选择性剪接（AS）在真核生物转录水平的基因表达调控中发挥着重要作用。通过基因的选择性剪接可以显着增加转录组和蛋白质组的灵活性和多样性。本研究利用脾脏和腹股沟淋巴结（ILN）等外周免疫器官的转录组数据来鉴定体内人工感染猪繁殖与呼吸综合征病毒（PRRSV）的抗PRRSV通城（TC）猪和PRRSV敏感大白（LW）猪之间的AS差异。结果表明，PRRSV感染诱导不同模式的全局选择性剪接事件（ASE）。其中，TC猪的脾脏和ILN中有373个基因和595个基因，而LW猪的脾脏和ILN中有458个基因和560个基因有显着差异的ASE。选择性剪接受到针对 PRRSV 感染的组织特异性和谱系特异性调节。丰富的GO术语和通路表明具有差异ASE的基因在转录调节、免疫反应、代谢和细胞凋亡中发挥重要作用。此外，与细胞凋亡相关的剪接因子SRSF4在LW猪中显着上调。通过 RT-PCR 和 DNA 测序验证了凋亡相关基因的功能分析。这些发现揭示了PRRSV抗性TC猪和PRRSV易感LW猪之间在选择性剪接水平上对PRRSV的不同反应，表明AS与PRRSV疾病抗性之间的潜在关系。
Alternative splicing (AS) plays a significant role in regulating gene expression at the transcriptional level in eukaryotes. Flexibility and diversity of transcriptome and proteome can be significantly increased through alternative splicing of genes. In the present study, transcriptome data of peripheral immune organs including spleen and inguinal lymph nodes (ILN) were used to identify AS difference between PRRSV-resistant Tongcheng (TC) pigs and PRRSV-susceptible Large White (LW) pigs artificially infected with porcine reproductive and respiratory syndrome virus (PRRSV) in vivo. The results showed that PRRSV infection induced global alternative splicing events (ASEs) with different modes. Among them, 373 genes and 595 genes in the spleen and ILN of TC pigs, while 458 genes and 560 genes in the spleen and ILN of LW pigs had significantly differential ASEs. Alternative splicing was subject to tissue-specific and lineage-specific regulation in response to PRRSV infection. Enriched GO terms and pathways showed that genes with differential ASEs played important roles in transcriptional regulation, immune response, metabolism, and apoptosis. Furthermore, a splicing factor associated with apoptosis, SRSF4, was significantly upregulated in LW pigs. Functional analysis on apoptosis associated genes was validated by RT-PCR and DNA sequencing. These findings revealed different response to PRRSV between PRRSV-resistant TC pigs and PRRSV-susceptible LW pigs at the level of alternative splicing, suggesting the potential relationship between AS and disease resistance to PRRSV.
登录
查看更多内容
影响因子：
6.7
作者：
De Maio FA;Risso G;Iglesias NG;Shah P;Pozzi B;Gebhard LG;Mammi P;Mancini E;Yanovsky MJ;Andino R;Krogan N;Srebrow A;Gamarnik AV
通讯作者：
Gamarnik AV
影响因子：
14.9
作者：
Wang PH;Fung SY;Gao WW;Deng JJ;Cheng Y;Chaudhary V;Yuen KS;Ho TH;Chan CP;Zhang Y;Kok KH;Yang W;Chan CP;Jin DY
通讯作者：
Jin DY
影响因子：
3.7
作者：
Pröll MJ;Neuhoff C;Schellander K;Uddin MJ;Cinar MU;Sahadevan S;Qu X;Islam MA;Poirier M;Müller MA;Drosten C;Tesfaye D;Tholen E;Große-Brinkhaus C
通讯作者：
Große-Brinkhaus C
DOI：
--
发表时间：
2016
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.8
作者：
Sessions OM;Tan Y;Goh KC;Liu Y;Tan P;Rozen S;Ooi EE
通讯作者：
Ooi EE