NK Cells Detect Changes in Adaptive Immunity within Mouse Decidua from Gestation Day Eight
NK Cells Detect Changes in Adaptive Immunity within Mouse Decidua from Gestation Day Eight
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DOI：
10.1016/j.placenta.2009.04.001
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发表时间：
2009-06-01
期刊：
影响因子：
3.8
通讯作者：
Croy, B. A.
中科院分区：
文献类型：
--
作者：
Hatta, K.;van den Heuvel, M. J.;Croy, B. A.
在妊娠期（gd）7小鼠基底蜕膜低温切片中，活的人CD56+ CD16-外周血自然杀伤细胞（NK）在剪切力作用下与内皮细胞表达的配体特异性结合。在一系列的分析中，粘附在gd7组织上的细胞数量在男性中是恒定的，但在有生育能力的女性中却有周期性的变化，这表明在月经周期中，这种NK细胞亚群的功能个体归巢潜力会短暂增加。无论性别如何，当基质为妊娠后期的基底蜕膜时，来自单个供体的黏附细胞数量显著增加。在这里，我们报告了人血液中的CD56+ CD16- NK细胞在gd7基底蜕膜上以单细胞形式粘附，在gd8和gd9组织上以大簇形式粘附，提示抗原识别和淋巴细胞活化。我们询问小鼠基底蜕膜中的哪些细胞在CD56+ CD16-细胞中触发这种反应。利用转基因骨髓树突状细胞（mDC）表达增强黄色荧光蛋白（eYFP）的小鼠蜕膜，我们发现簇的形成与mDC的接触无关。使用来自淋巴小鼠的蜕膜显示出聚集行为需要基质淋巴细胞。通过使用含有NK细胞但缺乏T和B细胞的蜕膜，鉴定出蜕膜T和/或B淋巴细胞是gd7后以激活CD56+ CD16细胞聚集的方式改变的细胞。这个时间点恰好在小鼠螺旋动脉修饰之前，这些指示细胞检测到它涉及INK细胞功能调节中的适应性、个体免疫反应。2009爱思唯尔有限公司版权所有。
Viable human CD56+ CD16- peripheral blood Natural Killer (NK) cells show specific in vitro binding under shear forces to ligands expressed by endothelial cells in cryostat sections of gestation day (gd)7 mouse decidua basalis. In serial assays, numbers of cells adhering to gd7 tissue are constant for men but have cyclical variation for fertile women, suggesting a brief gain in functional decidual homing potential of this NK cell subset during the menstrual cycle. Regardless of gender, numbers of adhering cells from an individual donor, increase dramatically when the substrate is decidua basalis from a later gestational timepoint. Here, we report that human blood CD56+ CD16- NK cells which adhere as single cells over gd7 decidua basalis, adhere as large clusters over gd8 and gd9 tissues, suggestive of antigen recognition and lymphocyte activation. We asked which cells within mouse decidua basalis trigger this response in CD56+ CD16- cells. Using decidua from mice transgenic for myeloid dendritic cell (mDC) expression of enhanced yellow fluorescent protein (eYFP), we found cluster formation was independent of mDC contact. Use of decidua from alymphoid mice showed clustering behavior required substrate iymphocytes. By use of decidua containing NK cells but lacking T and B cells, decidual T and/or B lymphocytes were identified as the cells altered after gd7 in a manner that activates CD56+ CD16- cell clustering. This timepoint is just prior to mouse spiral arterial modification and its detection by these indicator cells implicates adaptive, decidual immune responses in the regulation of INK cell function. (C) 2009 Elsevier Ltd. All rights reserved.