Comparison of Hyposmolar and Hyperosmolar Effects on in Vitro Luteinizing Hormone Secretion by Anterior Pituitary Cells
Comparison of Hyposmolar and Hyperosmolar Effects on in Vitro Luteinizing Hormone Secretion by Anterior Pituitary Cells
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低渗和高渗对垂体前叶细胞体外黄体生成素分泌影响的比较
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
S. Maruta
中科院分区：
文献类型：
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作者：
M. Greer;S. Greer;Zuzana Opsahl;S. Maruta
摘要 先前已经描述过，用低渗介质灌注急剧分散的腺垂体细胞会导致黄体生成素 (LH) 分泌立即高振幅“开启”爆发。在本报告中，进行了高渗溶液的逆向研究。高渗介质灌注可抑制 LH 分泌；恢复等渗引起 LH 分泌立即高幅度“关闭”爆发，与低渗灌注引起的情况非常相似。这些数据进一步支持了这样的理论：胞吐分泌可能涉及外细胞膜的扩张，从而将分泌颗粒吸引到细胞表面，在那里其内容物被挤出。
Abstract It has previously been described that perifusion of acutely dispersed adenohypophyseal cells with hypotonic medium causes an immediate high-amplitude “on” burst of luteinizing hormone (LH) secretion. In the present report the converse study with hyperosmolar solutions has been made. Perifusion with hypertonic medium depressed LH secretion; return to isotonicity caused an immediate high-amplitude “off” burst of LH secretion closely resembling that induced by hypotonic perifusion. The data give further support to the theory that exocytotic secretion may involve expansion of the outer cell membrane, thus drawing secretory granules to the cell surface where their contents are extruded.