The NAC Protein from Tamarix hispida, ThNAC7, Confers Salt and Osmotic Stress Tolerance by Increasing Reactive Oxygen Species Scavenging Capability
The NAC Protein from Tamarix hispida, ThNAC7, Confers Salt and Osmotic Stress Tolerance by Increasing Reactive Oxygen Species Scavenging Capability
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DOI：
10.3390/plants8070221
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发表时间：
2019-07-01
期刊：
影响因子：
4.5
通讯作者：
Ji, Xiaoyu
中科院分区：
文献类型：
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作者：
He, Zihang;Li, Ziyi;Ji, Xiaoyu
植物特异的NAC(NAM、ATAF1/2和CUC2)转录因子在响应非生物胁迫中起着重要作用。在本研究中，我们从刚毛柳中鉴定并鉴定了一种NAC蛋白ThNAC7。ThNAC7是一种核定位蛋白，具有转录激活活性。盐胁迫和渗透胁迫均能显著诱导ThNAC7的表达。获得了过表达ThNAC7(OE)和RNA干扰(RNAi)沉默的ThNAC7的瞬时转化植株，通过功能的获得和丧失来研究非生物的抗逆性。在瞬时转化和稳定转化的拟南芥中，过表达ThNAC7表现出对活性氧(ROS)清除能力和脯氨酸含量的增加，这是通过提高超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性来实现的。此外，ThNAC7还通过调节P5CS、SOD和POD基因的转录水平来激活这些生理变化。对野生型和ThNAC7转化拟南芥的RNA序列分析表明，ThNAC7可能调控了40多个已知的耐盐基因，其中包括胁迫相关基因和Tf基因。结果表明，ThNAC7通过增加渗透势和增强ROS清除作用，诱导与胁迫相关基因的转录水平，从而提高植物对盐胁迫和渗透胁迫的耐受性。
Plant specific NAC (NAM, ATAF1/2 and CUC2) transcription factors (TFs) play important roles in response to abiotic stress. In this study, we identified and characterized a NAC protein, ThNAC7, from Tamarix hispida. ThNAC7 is a nuclear localized protein and has transcriptional activation activity. ThNAC7 expression was markedly induced by salt and osmotic stresses. Transiently transformed T. hispida seedlings overexpressing ThNAC7 (OE) or with RNA interference (RNAi) silenced ThNAC7 were generated to investigate abiotic stress tolerance via the gain- and loss- of function. Overexpressing ThNAC7 showed an increased reactive oxygen species (ROS) scavenging capabilities and proline content, which was accomplished by enhancing the activities of superoxide dismutase (SOD) and peroxidase (POD) in transiently transformed T. hispida and stably transformed Arabidopsis plants. Additionally, ThNAC7 activated these physiological changes by regulating the transcription level of P5CS, SOD and POD genes. RNA-sequencing (RNA-seq) comparison between wild-type and ThNAC7-transformed Arabidopsis showed that more than 40 known salt tolerance genes might regulated by ThNAC7, including stress tolerance-related genes and TF genes. The results indicated that ThNAC7 induces the transcription level of genes associated with stress tolerance to enhance salt and osmotic stress tolerance via an increase in osmotic potential and enhanced ROS scavenging.