A Standardized Synthetic Eucalyptus Transcription Factor and Promoter Panel for Re-engineering Secondary Cell Wall Regulation in Biomass and Bioenergy Crops
A Standardized Synthetic Eucalyptus Transcription Factor and Promoter Panel for Re-engineering Secondary Cell Wall Regulation in Biomass and Bioenergy Crops
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DOI：
10.1021/acssynbio.8b00440
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发表时间：
2019-02-01
影响因子：
4.7
通讯作者：
Deutsch, Samuel
中科院分区：
文献类型：
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作者：
Hussey, Steven G.;Grima-Pettenati, Jacqueline;Deutsch, Samuel
调节次生细胞壁形成的转录网络的再工程可能允许在广泛种植的种植园作物如桉树中改善植物生物量。然而，目前缺乏可免费获得的标准化生物部件（例如，Phytobricks）与来自林木的IIS型组装方法兼容，并且需要加速非模型生物质作物中的转录网络推断。在这里，我们描述了一个多功能的三面板生物部件集合的设计和合成的221个次生细胞壁相关的巨桉转录因子编码序列和65个启动子，兼容GATEWAY，金门，MoClo，和GoldenBraid DNA组装方法，一般符合公认的Phytobrick语法。这种免费提供的资源旨在加速合成生物学在多种植物生物质作物中的应用，并使用高通量测定法（如DNA亲和纯化测序（DAP-seq）和增强的酵母单杂交（eY 1H）筛选）重建次生细胞壁转录网络。
Re-engineering of transcriptional networks regulating secondary cell wall formation may allow the improvement of plant biomass in widely grown plantation crops such as Eucalyptus. However, there is currently a scarcity of freely available standardized biological parts (e.g., Phytobricks) compatible with Type IIS assembly approaches from forest trees, and there is a need to accelerate transcriptional network inference in nonmodel biomass crops. Here we describe the design and synthesis of a versatile three-panel biological parts collection of 221 secondary cell wall-related Eucalyptus grandis transcription factor coding sequences and 65 promoters that are compatible with GATEWAY, Golden Gate, MoClo, and GoldenBraid DNA assembly methods and generally conform to accepted Phytobrick syntaxes. This freely available resource is intended to accelerate synthetic biology applications in multiple plant biomass crops and enable reconstruction of secondary cell wall transcriptional networks using high-throughput assays such as DNA affinity purification sequencing (DAP-seq) and enhanced yeast one-hybrid (eY1H) screening.