LINE-1 retrotransposition in human embryonic stem cells
LINE-1 retrotransposition in human embryonic stem cells
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DOI：
10.1093/hmg/ddm105
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发表时间：
2007-07-01
影响因子：
3.5
通讯作者：
Moran, John V.
中科院分区：
文献类型：
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作者：
Garcia-Perez, Jose L.;Marchetto, Maria C. N.;Moran, John V.
LINE-1元素约占人类DNA的17%，它们的移动性继续影响着基因组的进化。然而，对于能够支持LINE-1反转录转位的非转化细胞类型知之甚少。在这里，我们证明了人胚胎干细胞表达内源性的LINE-1元件，并且可以在体外进行LINE-1逆转录转位。由此产生的反转录转位事件可能发生在基因中，并可能导致靶位点基因组DNA的缺失。因此，这些数据表明LINE-1逆转录事件可能发生在人类发育的早期阶段。
LINE-1 elements comprise approximately 17% of human DNA and their mobility continues to impact genome evolution. However, little is known about the types of non-transformed cells that can support LINE-1 retrotransposition. Here, we show that human embryonic stem cells express endogenous LINE-1 elements and can accommodate LINE-1 retrotransposition in vitro. The resultant retrotransposition events can occur into genes and can result in the concomitant deletion of genomic DNA at the target site. Thus, these data suggest that LINE-1 retrotransposition events may occur during early stages of human development.