Fabrication and biocompatibility of novel bilayer scaffold for skin tissue engineering applications
Fabrication and biocompatibility of novel bilayer scaffold for skin tissue engineering applications
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DOI：
10.1177/0885328211416527
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发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Lee, Byong-Taek
中科院分区：
文献类型：
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作者：
Franco, Rose Ann;Min, Young-Ki;Lee, Byong-Taek
在这项研究中，一种新型的双层支架组成的电纺聚己内酯和聚（乳酸-共-羟基乙酸）（PCL/PLGA）膜和戊二醛（3.5%v/v）交联壳聚糖/明胶水凝胶使用两种方法：电纺膜上的冻干水凝胶（BS-1）和膜衬和铸造法（BS-2）。用扫描电子显微镜（SEM）观察支架的形态。机械强度，孔隙率，溶胀能力，和生物降解率的支架也进行了表征。通过细胞毒性评价材料的体外生物相容性，并采用MTT法测定材料上的细胞增殖。此外，通过SEM研究了细胞在材料上的粘附。将BS-2移植到Sprague-Dawley大鼠体内，以确定其体内行为和生物相容性。实验结果表明，向水凝胶中添加膜层降低了溶胀和降解速率，并在植入过程中提供了易于处理。移植的BS-2显示正常的伤口愈合，没有观察到严重的炎症反应。
In this study, a novel bilayer scaffold composed of electrospun polycaprolactone and poly(lacto-co-glycolic acid) (PCL/PLGA) membrane and glutaraldehyde (3.5% v/v) cross-linked chitosan/gelatin hydrogel was fabricated using two methods: electrospinning of the membrane onto the lyophilized hydrogel (BS-1) and membrane underlaying and casting method (BS-2). The morphology of the fabricated scaffolds was examined by scanning electron microscope (SEM). Mechanical strength, porosity, swelling capacity, and biodegradation rates of the scaffolds were also characterized. The in vitro biocompatibility of the materials was investigated by assessing cytotoxicity and cell proliferation on the material was measured using MTT assay. In addition, cell adhesion on the material was investigated by SEM. The BS-2 was grafted in Sprague-Dawley rats to determine its in vivo behavior and biocompatibility. The experimental results showed that the addition of the membrane layer to the hydrogel decreased swelling and degradation rates and provided ease of handling during implantation. Grafted BS-2 showed normal wound healing and no major inflammatory reaction was observed.