Effects of combined administration of transforming growth factor-β1 and epidermal growth factor on properties of the in situ frozen anterior cruciate ligament in rabbits
Effects of combined administration of transforming growth factor-β1 and epidermal growth factor on properties of the in situ frozen anterior cruciate ligament in rabbits
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DOI：
10.1016/s0736-0266(02)00065-7
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发表时间：
2002-11-01
影响因子：
2.8
通讯作者：
Frank, CB
中科院分区：
文献类型：
--
作者：
Sakai, T;Yasuda, K;Frank, CB
前交叉韧带（ACL）重建术中使用的自体肌腱移植物的力学性能在手术后降低。以前的研究表明，生长因子如转化生长因子-β 1（TGF-β 1）和表皮生长因子（EGF）可以刺激成纤维细胞增殖，并增加这些细胞的胶原和非胶原蛋白的合成。这些因素可能是有用的，因此，在防止移植后退化或加速退化的移植物的机械恢复。因此，我们的研究的目的，是澄清TGF-β 1和EGF的生物技术性能的影响，使用原位冻融ACL模型在兔。共142只家兔接受了右侧ACL和ACL的冻融处理。然后被分成四组。第I组作为冻融对照，但未进行其他处理。在第II组中，仅应用递送载体（纤维蛋白封闭剂）。在第三组中，应用4 ng TGF-β 1和100 ng EGF与溶剂混合。在第IV组中，将较高剂量（2 μ g TGF-β 1和50 μ g EGF）的生长因子与溶剂混合。12周后，根据机械性能、含水量、组织学和超微结构观察结果对各组进行比较。第III组的横截面积（平均7.1 mm（2））显著小于第I、II和IV组（9.0、8、2、3、4、5、6、7、7、8、8、9、9、10、11、12、12、13、14、15、16、17、18、18、19、19、和9.4 mm（2））。第12周时，组III的拉伸强度（62.2 MPa）显著大于组I、II和IV（分别为35.6、43.7和36.9 MPa），而组III的含水量。(70.7%）显著低于I组（75.2%）。第I、II和IV组之间无其他显著差异。在组I和组III中观察到胶原纤维直径的单峰分布，而在组III中观察到双峰分布。本研究表明，低剂量应用TGF-β 1和EGF不仅显著抑制了含水量和横截面积的增加，而且还抑制了拉伸强度的降低。然而，高剂量的TGF-β 1和EGF并不具有相同的有益效果。(C)2002年。骨科研究协会。由爱思唯尔科技有限公司出版。保留所有权利。
The mechanical properties of tendon autografts used in reconstruction of the anterior cruciate ligament (ACL) are reduced after surgery. Previous studies showed that growth factors such as transforming growth factor-beta1 (TGF-beta1) and epidermal growth factor (EGF) can stimulate fibroblast proliferation and increase collagen and noncollagenous protein synthesis by these cells. These factors might be useful, therefore, in preventing graft deterioration after transplantation or accelerating mechanical restoration of the deteriorated graft. The purpose of our study, therefore, was to clarify the effects of TGF-beta1 and EGF on biontechanical properties using an in situ freeze-thaw ACL model in the rabbit. A total of 142 rabbits underwent the freeze-thaw treatment in the right ACL and. were then divided into four groups. Group I served as a freeze-thaw, but otherwise untreated control, In Group II, a delivery vehicle (fibrin sealant) alone was applied. In Group III, 4-ng TGF-beta1 and 100-ng EGF mixed with the vehicle were applied. In pared at Group IV, higher doses (2-mug TGF-beta1 and 50-mug EGF) of growth factors were mixed with the vehicle. The groups were compared 12 weeks on the basis of mechanical properties, water content, and histological and ultrastructural observations, The cross-sectional sectional area of Group Ill (average, 7.1 mm(2)) was significantly less than that of Groups I, II, and IV (9.0, 8,2., and 9.4 mm(2) respectively) at 12 weeks. The tensile strength of Group III (62.2 MPa) was significantly greater than that of Groups I, II, and IV (35.6, 43.7, and 36.9 MPa, respectively) at 12 weeks, while the water content of Group III. (70.7%) was significantly lower than that of Group I (75.2%). No other significant differences occurred among Groups I, II, and IV. A unimodal distribution of collagen fibril diameters was noted in Groups I and , while a bimodal pattern was found in Group Ill. This study demonstrated that low dose application of TGF-beta1 and EGF significantly inhibited not only the increased water content and cross-sectional area, but also the decreased tensile strength. caused by the freeze-thaw treatment, while a high dose of TGF-beta1 and EGF does not have the same beneficial effects. (C) 2002. Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.