Genetic characterization of contemporary wild-type measles viruses from Vietnam and the People's Republic of China: identification of two genotypes within clade H
Genetic characterization of contemporary wild-type measles viruses from Vietnam and the People's Republic of China: identification of two genotypes within clade H
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DOI：
10.1016/s0168-1702(01)00268-4
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发表时间：
2001-09-01
期刊：
影响因子：
5
通讯作者：
Rota, PA
中科院分区：
文献类型：
--
作者：
Liffick, SL;Thoung, NT;Rota, PA
对1998年在越南河内附近分离的17种野生型麻疹病毒以及1995年、1998年和1999年在中华人民共和国湖南、海南、山东和安徽省分离的8种病毒进行了基因表征。先前的研究表明，与世界其他地区发现的野生型麻疹病毒相比，来自中国的野生型病毒在遗传上是不同的，并组成了一个新的病毒进化枝，即进化枝H。在这项研究中，对编码核蛋白（N）COOH末端150个氨基酸和整个血凝素（H）蛋白的核苷酸进行的序列分析表明，尽管所有来自越南的病毒都是进化枝H的成员，但它们与中国病毒明显不同。除了MVi/北京。China /94/1，越南病毒与所有中国病毒在N和H基因的核苷酸水平上分别至少有3.5%和2.5%的差异。这些数据表明，进化枝H应分为两个基因型，中国病毒的基因型H1和越南病毒的基因型H2。从中国或越南输入美国的麻疹病毒的序列分析表明，这种基因型的指定将有助于未来的麻疹监测活动。(C)2001爱思唯尔科技有限公司。保留所有权利。
Genetic characterization was conducted on 17 wild-type measles viruses isolated near Hanoi, Vietnam, during 1998 as well as on eight viruses isolated in the Hunan, Hainan, Shandong, and Anhui provinces of the People's Republic of China during 1995, 1998, and 1999. Previous studies had shown that, compared to wild-type measles viruses found in other parts of the world, wild-type viruses from China were genetically distinct and comprised a new clade of viruses, clade H. In this study, sequence analyses of the nucleotides coding for the COOH terminal 150 amino acids of the nucleoprotein (N) and the entire hemagglutinin (H) protein indicated that although all of the viruses from Vietnam were members of clade H, they were clearly distinct from the Chinese viruses. With the exception of MVi/Beijing. China /94/1, the Vietnamese viruses differed from all of the Chinese viruses by at least 3.5 and 2.5% at the nucleotide level for the N and H genes, respectively. These data suggest that clade H should be divided into two genotypes with the Chinese viruses placed in genotype HI and the Vietnamese viruses in genotype H2. Sequence analysis of measles viruses imported into the United States from either China or Vietnam demonstrated that this designation of genotypes will be helpful in future measles surveillance activities. (C) 2001 Elsevier Science B.V. All rights reserved.