Chemotactic peptide receptor modulation in polymorphonuclear leukocytes.
Chemotactic peptide receptor modulation in polymorphonuclear leukocytes.
复制标题
多形核白细胞中的趋化肽受体调节。
DOI：
10.1083/jcb.85.3.703
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发表时间：
1980-06
影响因子：
7.8
通讯作者：
Zigmond, S H
中科院分区：
文献类型：
--
作者：
Sullivan, S J;Zigmond, S H
趋化肽 N-甲酰基正亮氨酰苯丙氨酸 (FNLLP) 与其在兔多形核白细胞 (PMN) 上的受体的结合可调节可用肽受体的数量。与 FNLLP 一起孵育会降低随后的结合能力，这种现象被称为受体下调。趋化肽受体的下调在受体损失的速率和程度方面均依赖于浓度。剂量反应与 FNLLP 与受体结合的反应相似。时间进程很快；即使FNLLP浓度低至3 x 10(-9) M，受体的新平衡浓度也会在15分钟内达到。下调取决于温度，但即使在 4 摄氏度也会发生。伴随着下调，一些肽变得不可逆地与细胞相关。在 4 摄氏度时，有少量不可解离的肽积累，并迅速达到稳定状态。在较高温度下，受体数量达到平衡后，不可解离的肽会继续积累，积累的量可超过受体初始数量多达 300%。 37 摄氏度时肽摄取的剂量反应反映了结合的剂量反应，表明它是受体介导的。这种摄取可能通过胞饮作用机制发生。尽管PMN不被认为具有胞饮作用，但添加FNLLP会导致胞饮作用速率四倍刺激（通过[3H]蔗糖的摄取来测量）。
The binding of the chemotactic peptide N- formylnorleucylleucylphenylalanine (FNLLP) to its receptor on rabbit polymorphonuclear leukocytes (PMNs) modulates the number of available peptide receptors. Incubation with FNLLP decreases subsequent binding capacity, a phenomenon that has been termed receptor down regulation. Down regulation of the chemotactic peptide receptor is concentration dependent in both the rate and extent of receptor loss. The dose response parallels that of FNLLP binding to the recptor. The time- course is rapid; even at concentrations of FNLLP as low as 3 x 10(-9) M, the new equilibrium concentration of receptors is reached within 15 min. Down regulation is temperature dependent, but does occur even at 4 degrees C. Concomitant with down regulation, some of the peptide becomes irreversibly cell associated. At 4 degrees C, there is a small accumulation of nondissociable peptide that rapidly reaches a plateau. At higher temperatures, accumulation of nondissociable peptide continues after the rceptor number has reached equilibrium, and the amount accumulated can exceed the initial number of receptors by as much as 300%. The dose response of peptide uptake at 37 degrees C reflects that of binding, suggesting that it is receptor mediated. This uptake may occur via a pinocytosis mechanism. Although PMNs have not been considered to be pinocytic, the addition of FNLLP causes a fourfold stimulation of the rate of pinocytosis as measured by the uptake of [3H]sucrose.