ENHANCED HEMATOPOIETIC ACTIVITY OF A HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR INTERLEUKIN-3 FUSION PROTEIN
ENHANCED HEMATOPOIETIC ACTIVITY OF A HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR INTERLEUKIN-3 FUSION PROTEIN
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DOI：
10.1073/pnas.88.13.5809
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发表时间：
1991-07-01
影响因子：
11.1
通讯作者：
PARK, LS
中科院分区：
文献类型：
--
作者：
CURTIS, BM;WILLIAMS, DE;PARK, LS
Granulocyte/macrophage colony-stimulating factor-interleukin 3 (GM-CSF-IL-3) fusion proteins were generated by construction of a plasmid in which the coding regions of human GM-CSF and IL-3 cDNAs were connected by a synthetic linker sequence followed by subsequent expression in yeast. Both GM-CSF-IL-3 and IL-3-GM-CSF fusion proteins were purified to homogeneity and shown to bind to cell-surface receptors through either their GM-CSF or IL-3 domains. The fusion proteins exhibited enhanced receptor affinity, proliferative activity, and hematopoietic colony-stimulating activity compared with either IL-3 and/or GMCSF alone. This suggests that GM-CSF-IL-3 fusion proteins may hold future promise as therapeutic agents.
