Reproductive failure and the free amino acid pools in pea aphids (Acyrthosiphon pisum) lacking symbiotic bacteria
Reproductive failure and the free amino acid pools in pea aphids (Acyrthosiphon pisum) lacking symbiotic bacteria
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DOI：
10.1016/0022-1910(95)00105-0
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发表时间：
1996-03-01
影响因子：
2.2
通讯作者：
Douglas, AE
中科院分区：
文献类型：
--
作者：
Douglas, AE
豌豆蚜 (Acyrthosiphon pisum) 在出生时接受抗生素利福平处理，以破坏共生细菌（即无性共生蚜虫）生长非常缓慢，无性共生蚜虫中胚胎的生长尤其受到抑制，以至于到了成年期，胚胎部分占共生蚜虫中总蛋白质的 65%，但仅占无共生蚜虫中蛋白质的 12%，概况分析对蚜虫及其胚胎游离氨基酸库的分析表明，芳香族氨基酸苯丙氨酸和色氨酸在无共生体胚胎中的浓度非常低，推测这些氨基酸可能限制无共生蚜虫胚胎的生长，也有迹象表明赖氨酸可能限制4日龄幼虫无共生体母体组织的生长，而蛋氨酸可能在较老的无性共生蚜虫供应短缺。
Pea aphids (Acyrthosiphon pisum) treated at birth with the antibiotic, rifampicin, to disrupt the symbiotic bacteria (i.e, aposymbiotic aphids) grow very slowly, The growth of embryos in the aposymbiotic aphids was particularly depressed, such that, by adulthood, the embryo fraction represented 65% of the total protein in symbiotic aphids, but just 12% of the protein in aposymbionts, Profile analysis of the free amino acid pools of the aphids and their embryos indicated that the concentrations of the aromatic amino acids phenylalanine and tryptophan were very low in the embryos of aposymbionts, and it is proposed that these amino acids may limit embryo growth in aposymbiotic aphids, There are also indications that lysine may be limiting to the growth of the maternal tissues of 4-day-old larval aposymbionts, and methionine may be in short supply in older aposymbiotic aphids.