IMMOBILIZED PEROXIDASE : A POTENTIAL BIOINDICATOR FOR EVALUATION OF THERMAL PROCESSES

IMMOBILIZED PEROXIDASE : A POTENTIAL BIOINDICATOR FOR EVALUATION OF THERMAL PROCESSES
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固定化过氧化物酶:用于评估热过程的潜在生物指示剂

DOI:
10.1111/j.1365-2621.1991.tb05326.x
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发表时间:
1991
影响因子:
3.9
通讯作者:
P. Tobback
P. Tobback
中科院分区:
农林科学3区
文献类型:
--
作者:
Z. Weng;M. Hendrickx;G. Maesmans;P. Tobback

文献摘要

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研究了十二烷环境中多孔玻璃珠上/内共价固定的过氧化物酶的热稳定性部分,作为评估巴氏灭菌条件下热过程的生物指示剂。经验证的生物指示剂的 z 值为 10.1°C,Dref(70°C)为 22 分钟。在 70°C 和 80°C 的处理温度下,从生物指示剂读取的致死率 (F)bio 与根据一般方法从时间温度数据计算出的目标 Ft10.0 值非常吻合。
The heat-stable fraction of covalently immobilized peroxidase on/in porous glass beads in an environment of dodecane was studied as a bioindicator for evaluation of thermal processes under conditions of pasteurization. The verified bioindicator had a z value of 10.1°C and a Dref (at 70°C) of 22 min. At processing temperatures of 70°C and 80°C, the lethalities (F)bio as read from the bioindicator agreed very well with the target Ft10.0 values calculated from the time temperature data according to the general method.