Annexin A5-conjugated quantum dots with a paramagnetic lipidic coating for the multimodal detection of apoptotic cells
Annexin A5-conjugated quantum dots with a paramagnetic lipidic coating for the multimodal detection of apoptotic cells
复制标题
DOI：
10.1021/bc0600463
复制
发表时间：
2006-07-19
影响因子：
4.7
通讯作者：
Strijkers, Gustav J.
中科院分区：
文献类型：
--
作者：
van Tilborg, Geralda A. F.;Mulder, Willem J. M.;Strijkers, Gustav J.
细胞凋亡，或程序性细胞死亡，在包括癌症在内的各种疾病的病因学中扮演着重要的角色。细胞凋亡的可视化将允许及早检测治疗效率和评估疾病进展。为此，我们开发了一种新型的膜联蛋白A5偶联双峰纳米粒子。这种纳米粒子由包裹在顺磁胶束中的量子点组成，以使其能够用于光学成像和磁共振成像。多个重组人Annexin A5蛋白分子共价偶联到纳米颗粒上进行靶向。在本研究中，通过荧光显微镜和核磁共振证实了膜联蛋白A5偶联纳米粒对细胞凋亡的特异性，这证实了其在体内两种成像方式下检测细胞凋亡的潜力。
Apoptosis, or programmed cell death, plays an important role in the etiology of a variety of diseases, including cancer. Visualization of apoptosis would allow both early detection of therapy efficiency and evaluation of disease progression. To that aim we developed a novel annexin A5-conjugated bimodal nanoparticle. The nanoparticle is composed of a quantum dot that is encapsulated in a paramagnetic micelle to enable its use both for optical imaging and MRI. Multiple recombinant human annexin A5 protein molecules were covalently coupled to the nanoparticle for targeting. In this study the specificity of the annexin A5-conjugated nanoparticles for apoptotic cells was demonstrated both with fluorescence microscopy and MRI, which confirms its potential for the detection of apoptosis with both imaging modalities in vivo.