Innate and acquired immune responses against Candida albicans in congenic B10.D2 mice with deficiency of the C5 complement component.
Innate and acquired immune responses against Candida albicans in congenic B10.D2 mice with deficiency of the C5 complement component.
复制标题
缺乏 C5 补体成分的同源 B10.D2 小鼠针对白色念珠菌的先天和获得性免疫反应。
DOI：
10.1080/02681218680000551
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Domer,JE
中科院分区：
文献类型：
--
作者：
Lyon,FL;Hector,RF;Domer,JE
同种小鼠，足够或缺乏的补体C5成分，评价其先天性和获得性免疫应答白色念珠菌。当未免疫的小鼠通过静脉注射进行攻击，并间隔一段时间处死以进行肾脏培养分析时，很明显，C5充足的小鼠能够更有效地处理C。在攻击后的第一周内，白色念珠菌比C5缺陷型小鼠更明显。当免疫小鼠静脉内攻击，以评估保护性反应的发展，一个完整的补体级联似乎有助于更快的清除真菌在最初几周内的挑战，但到第四周后的挑战，真菌的数量显着下降，在这两种类型的小鼠，并在水平没有显着差异。在C5-充足和C5-缺乏小鼠之间的迟发型超敏反应或迟发型超敏反应特异性抗体的发展中也没有检测到显著差异。C5缺陷小鼠的水平确实略高于C5小鼠，但这可能只是反映了免疫和非免疫动物静脉内激发后前3周内抗原负荷延长。迟效补体成分虽然对C.在非免疫动物中的白念珠菌对特异性免疫应答的发展没有不利影响，因为两组之间的延迟过敏反应是等效的，抗体应答没有显著改变，并且免疫动物中的攻击的最终结果没有受到影响。
Congenic mice, sufficient or deficient with respect to the C5 component of complement, were evaluated for their innate and acquired immune responses toCandida albicans. When unimmunized mice were challenged intravenously and sacrificed at intervals for cultural analyses of kidneys, it was clear that C5-sufficient mice were able to deal more effectively withC. albicansduring the first week after challenge than C5-deficient mice. When immunized mice were challenged intravenously to assess the development of protective responses, an intact complement cascade appeared to contribute to the more rapid clearance of fungi during the first few weeks following challenge, but by the fourth week after challenge, the numbers of fungi had decreased significantly in both types of mice and were at levels which were not significantly different. No significant differences were detected in the development of delayed hypersensitivity orCandida-specific antibody between C5-sufficient and C5-deficient mice either. C5-deficient mice did have slightly elevated levels over the C5 mice, but this may simply reflect the prolonged antigenic load during the first 3 weeks following intravenous challenge in both immune and nonimmune animals. The later-acting complement components, while appearing to contribute to the early inhibition of the growth ofC. albicansin the nonimmune animal, had no adverse effect on the development of specific immune responses, in that delayed hypersensitive responses were equivalent between the two groups, antibody response was not significantly altered and the ultimate outcome of challenge in immunized animals was not affected.