Measurement of the Ability of Cells to Infiltrate Normal Tissues In vitro
Measurement of the Ability of Cells to Infiltrate Normal Tissues In vitro
复制标题
体外细胞浸润正常组织能力的测量
作者：
D. Easty;G. Easty
鸡胚绒毛尿囊膜的器官培养已被用于建立一种定量测定各种正常细胞和肿瘤细胞浸润能力的方法。正常成纤维细胞、小鼠腹膜细胞和低致瘤性细胞的浸润较差且缓慢，而大多数肿瘤的浸润迅速。一些侵袭性更强的肿瘤细胞通过正常组织的迁移速度最低，为2-3 μm/h。一种倾向于在绒毛尿囊膜上形成聚集体的肿瘤细胞系引起了膜外胚层的明显排斥反应。秋水仙胺，它抑制细胞过程的形成和细胞的定向迁移，和二丁酰环AMP，它恢复某些方面的正常行为的肿瘤细胞在体外，对膜的肿瘤细胞的入侵几乎没有影响。
Organ cultures of chorioallantoic membranes of hen eggs have been used to establish a quantitative method of measuring the infiltrative ability of a variety of normal and tumour cells. Normal fibroblasts, mouse peritoneal cells and cells of low tumorigenicity infiltrated poorly and slowly whereas most tumours infiltrated rapidly. Some cells of the more invasive tumours achieved minimum rates of migration through the normal tissue of 2-3 μm/h. One tumour line which tended to form aggregates on the chorioallantoic membrane elicited a pronounced rejection response from the ectoderm of the membrane. Colcemid, which inhibits the formation of cell processes and the directional migration of cells, and dibutyryl cyclic AMP, which restores certain aspects of normal behaviour to tumour cells in vitro, had little effect on the invasion of the membrane by tumour cells.