Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers.
Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers.
复制标题
速激肽信号抑制蜜蜂工蜂任务特异性行为反应。
作者：
Han B;Wei Q;Wu F;Hu H;Ma C;Meng L;Zhang X;Feng M;Fang Y;Rueppell O;Li J
行为专业化是群居昆虫成功的关键，并导致群体成员之间的劳动分工。对任务特异性刺激的反应阈值被认为是近端调节行为专业化，但其神经生物学调节是复杂的，尚未得到很好的理解。在这里，我们证明了任务相关刺激的反应阈值与蜜蜂工蜂的三种行为表型的专业化相对应。定量神经肽球比较表明，两种速激肽相关肽（TRP2和TRP3）可作为改变这些反应阈值的候选肽。基于我们对其受体结合和下游信号传导的表征，我们证实了速激肽信号传导在调节蜜蜂工蜂特异性反应中的功能作用：TRP2注射和rnai介导的下调对每种行为特异性表型的任务特异性刺激的反应性产生一致的相反影响，但对与其任务无关的刺激却没有影响。因此，我们的研究表明，TRP信号调节专业化蜜蜂工蜂的任务特异性反应程度，并可能更普遍地控制动物行为的情境特异性。
Behavioral specialization is key to the success of social insects and leads to division of labor among colony members. Response thresholds to task-specific stimuli are thought to proximally regulate behavioral specialization, but their neurobiological regulation is complex and not well understood. Here, we show that response thresholds to task-relevant stimuli correspond to the specialization of three behavioral phenotypes of honeybee workers in the well-studied and important Apis mellifera and Apis cerana. Quantitative neuropeptidome comparisons suggest two tachykinin-related peptides (TRP2 and TRP3) as candidates for the modification of these response thresholds. Based on our characterization of their receptor binding and downstream signaling, we confirm a functional role of tachykinin signaling in regulating specific responsiveness of honeybee workers: TRP2 injection and RNAi-mediated downregulation cause consistent, opposite effects on responsiveness to task-specific stimuli of each behaviorally specialized phenotype but not to stimuli that are unrelated to their tasks. Thus, our study demonstrates that TRP signaling regulates the degree of task-specific responsiveness of specialized honeybee workers and may control the context specificity of behavior in animals more generally.