Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity
Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity
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DOI：
10.1016/s0014-5793(00)01356-9
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发表时间：
2000-04-07
期刊：
影响因子：
3.5
通讯作者：
Wang, J
中科院分区：
文献类型：
--
作者：
Wang, XL;Sim, AS;Wang, J
我们探讨了33例产后胎盘中内皮型一氧化氮合酶（eNOS）基因型和吸烟对蛋白质水平和酶活性的相互作用。而在罕见等位基因中eNOS蛋白水平较低，（0.48 ± 0.11，n = 9 vs.1.05 ± 0.10，n = 24，P < 0.01），在稀有等位基因中eNOS酶活性高约7倍（4556.2 +/- 255.4 vs. 621.8 +/- 180.5 cpm/mg/min，P < 0.01）。吸烟者的eNOS蛋白水平较低（1.07 ± 0.09vs.0.50 ± 0.19，P < 0.05），仅在稀有等位基因中降低eNOS活性（不吸烟者：6143.8 +/- 251.2，n = 5，吸烟者：2968.5 +/- 259.4，n = 4，减少52%，P < 0.01），我们得出结论，eNOS多态性与蛋白水平和酶活性之间的关联可通过吸烟改变，吸烟的影响取决于eNOS基因型，（C）2000欧洲生物化学学会联合会。
We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lo,ver in the rare allele (0.48 +/- 0.11, n = 9 vs. 1.05 +/- 0.10, n = 24, P < 0.01), the eNOS enzyme activity was about 7-fold higher in the rare allele (4556.2 +/- 255.4 vs. 621.8 +/- 180.5 cpm/mg/min, P < 0.01). Smokers had lower eNOS protein levels (1.07 +/- 0.09 vs. 0.50 +/- 0.19, P < 0.05) in both alleles, It reduced the eNOS activities only in the rare allele (non-smokers: 6143.8 +/- 251.2, n = 5, smokers: 2968.5 +/- 259.4, n = 4, 52% reduction, P < 0.01), We conclude that associations between eNOS polymorphism and protein levels and enzyme activities are modifiable by smoking, the effects of smoking are dependent on the eNOS genotypes, (C) 2000 Federation of European Biochemical Societies.