Antimutagenic effect of Agaricus blazei Murrill mushroom on the genotoxicity induced by cyclophosphamide
Antimutagenic effect of Agaricus blazei Murrill mushroom on the genotoxicity induced by cyclophosphamide
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DOI：
10.1016/s1383-5718(01)00228-5
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发表时间：
2001-09-20
影响因子：
1.9
通讯作者：
Ribeiro, LR
中科院分区：
文献类型：
--
作者：
Delmanto, RD;de Lima, PLA;Ribeiro, LR
姬松茸提取物先前已被证明具有抗癌和抗诱变特性。这些结果提示，除调节免疫系统外，blazei的抗诱变活性可能参与了其抗癌作用。为了探讨blazei在体内可能的抗诱变作用，我们采用骨髓微核试验（MNPCE）和外周血微核试验（MNRET）评价了blazei对小鼠环磷酰胺（CP）诱导的致裂性的影响。雄性瑞士小鼠在4度、21度和60度三种不同的温度下接受CP（25或50 mg/kg i.p.p）或CP加蘑菇溶液治疗。不同品种蘑菇混合水溶液抑制小鼠骨髓和外周血中CP对微核的诱导作用。与混合谱系相比，单个分离的谱系不会导致小鼠骨髓或血细胞中cp诱导的MN频率降低。结果表明，在某些情况下，这些蘑菇表现出抗诱变活性，可能有助于抗癌作用。(C) 2001 Elsevier Science B.V.版权所有
Agaricus blazei Murrill extracts have previously been shown to have anticarcinogenic and antimutagenic proper-ties. These results suggest that antimutagenic activity, besides the modulation of the immune system, might be involved in the anticarcinogenic action of A. blazei. To investigate the possible antimutagenic effect of A. blazei in vivo, we evaluated its effect on clastogenicity induced by cyclophosphamide (CP) in mice, using the micronucleus test in bone marrow (MNPCE) and in peripheral blood (MNRET). Male Swiss mice were treated with CP (25 or 50 mg/kg i.p.) or with CP plus mushroom solution at three different temperatures: 4, 21, and 60 degreesC. Aqueous solution of a mixture from various lineages of the mushroom inhibited induction of micronuclei by CP in bone marrow and in peripheral blood of mice. In contrast to the mixture of lineages, a single isolated lineage did not lead to a reduction of CP-induced MN frequencies in either bone marrow or blood cells of mice. The results suggest that under certain circumstances these mushrooms exhibit antimutagenic activities that might contribute to an anticarcinogenic effect. (C) 2001 Elsevier Science B.V. All rights reserved.