Cell kinetics during regeneration in the sponge Halisarca caerulea: how local is the response to tissue damage?
Cell kinetics during regeneration in the sponge Halisarca caerulea: how local is the response to tissue damage?
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作者：
Alexander BE;Achlatis M;Osinga R;van der Geest HG;Cleutjens JP;Schutte B;de Goeij JM
海绵有一个显著的能力，迅速再生，以应对伤口造成。此外，海绵迅速更新其过滤系统（胆黄细胞），以维持健康的细胞群。本研究描述了在实验伤口造成后早期（0-8小时），包覆礁海绵海小囊中胆鞘细胞的细胞动力学。随后，我们研究了直接靠近伤口、距离伤口1cm和3cm的6天内再生和细胞增殖之间的空间关系。5-溴-2 ' -脱氧尿苷（BrdU）的掺入决定了细胞的增殖。我们证明，在早期再生过程中，与稳定状态下未损伤组织（46.6±2.6%）相比，伤口附近的肝细胞生长比例（即增殖细胞的百分比）减少了（7.0±2.5%），而细胞周期的长度仍然很短（5.6±3.4小时）。随着时间的推移，所有区域的增殖胆管细胞的百分比都增加了，再生6天后，胆管细胞的增殖率与稳态组织相当。离创面较远的组织区域比离创面较近的组织区域有更高的心肌细胞增殖率，这表明创面附近的组织需要更多的资源。再生海绵与稳态海绵的间质细胞增殖数量无显著差异。我们的数据表明，产生富含胶原蛋白的创面组织是毛囊菌组织再生的关键过程，有助于迅速占领创面暴露的裸露基质。再生和胆鞘细胞更新是相互竞争和负相关的生活史特征，对海绵的生存都至关重要。将有限的资源有效地分配到这些生活史性状上，使海绵的生态成功和多样化成为可能。
Sponges have a remarkable capacity to rapidly regenerate in response to wound infliction. In addition, sponges rapidly renew their filter systems (choanocytes) to maintain a healthy population of cells. This study describes the cell kinetics of choanocytes in the encrusting reef sponge Halisarca caerulea during early regeneration (0–8 h) following experimental wound infliction. Subsequently, we investigated the spatial relationship between regeneration and cell proliferation over a six-day period directly adjacent to the wound, 1 cm, and 3 cm from the wound. Cell proliferation was determined by the incorporation of 5-bromo-2′-deoxyuridine (BrdU). We demonstrate that during early regeneration, the growth fraction of the choanocytes (i.e., the percentage of proliferative cells) adjacent to the wound is reduced (7.0 ± 2.5%) compared to steady-state, undamaged tissue (46.6 ± 2.6%), while the length of the cell cycle remained short (5.6 ± 3.4 h). The percentage of proliferative choanocytes increased over time in all areas and after six days of regeneration choanocyte proliferation rates were comparable to steady-state tissue. Tissue areas farther from the wound had higher rates of choanocyte proliferation than areas closer to the wound, indicating that more resources are demanded from tissue in the immediate vicinity of the wound. There was no difference in the number of proliferative mesohyl cells in regenerative sponges compared to steady-state sponges. Our data suggest that the production of collagen-rich wound tissue is a key process in tissue regeneration for H. caerulea, and helps to rapidly occupy the bare substratum exposed by the wound. Regeneration and choanocyte renewal are competing and negatively correlated life-history traits, both essential to the survival of sponges. The efficient allocation of limited resources to these life-history traits has enabled the ecological success and diversification of sponges.
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影响因子：
64.8
作者：
Buczacki, Simon J. A.;Zecchini, Heather Ireland;Winton, Douglas J.
通讯作者：
Winton, Douglas J.
影响因子：
5.4
作者：
Boshuizen, JA;Reimerink, JHJ;Einerhand, AWC
通讯作者：
Einerhand, AWC
DOI：
10.1017/s0025315402005295
发表时间：
2002-02-01
影响因子：
1.2
作者：
Bell, JJ
通讯作者：
Bell, JJ
影响因子：
56.9
作者：
de Goeij, Jasper M.;van Oevelen, Dick;Admiraal, Wim
通讯作者：
Admiraal, Wim
影响因子：
2.6
作者：
HARRISON, FW
通讯作者：
HARRISON, FW