RESONANCE RAMAN-SPECTROSCOPY OF SPECIFICALLY [EPSILON-15N]LYSINE-LABELED BACTERIORHODOPSIN
RESONANCE RAMAN-SPECTROSCOPY OF SPECIFICALLY [EPSILON-15N]LYSINE-LABELED BACTERIORHODOPSIN
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DOI：
10.1073/pnas.78.3.1643
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
HERLIHY, WC
中科院分区：
文献类型：
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作者：
ARGADE, PV;ROTHSCHILD, KJ;HERLIHY, WC
通过将15 N特异性掺入ε-赖氨酸中来研究第二赖氨酸与细菌视紫红质（来自盐生盐杆菌（Halobacterium halobium））的亚视黄基席夫碱的可能相互作用。赖氨酸残基的氨基。比较100、0和50%标记赖氨酸上生长的细菌视紫红质的共振拉曼光谱表明，15 N同位素对席夫碱振动的影响可以通过仅在席夫碱氮上的15 N标记来解释。该数据还提供了视网膜发色团与ε-葡萄糖的连接的原位确认。赖氨酸的氨基氮。
The possible interaction of a 2nd lysine with the retinylidene Schiff base of bacteriorhodopsin (from Halobacterium halobium) was investigated by specific incorporation of 15N into the .epsilon.-amino groups of the lysine residues. Comparison of resonance Raman spectra of bacteriorhodopsin grown on 100, 0 and 50% labeled lysine demonstrates that 15N isotope effects on the Schiff base vibration can be accounted for by 15N labeling only at the Schiff base nitrogen. The data also provide in situ confirmation of the linkage of the retinal chromophore with the .epsilon.-amino nitrogen of lysine.