Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression
Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression
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DOI：
10.1182/blood-2006-03-006569
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发表时间：
2007-01-01
期刊：
影响因子：
20.3
通讯作者：
Baron, Margaret H.
中科院分区：
文献类型：
--
作者：
Fraser, Stuart T.;Isern, Joan;Baron, Margaret H.
原始红细胞是哺乳动物胚胎发育过程中形成的第一种造血细胞类型，出现在卵黄囊的血岛中。当卵黄囊和胚胎血管系统之间的连接成熟时，大的有核的EryP在中期左右开始循环。两到三天后，确定的红系小细胞(EryD)开始在胎肝内分化，并在循环中迅速超过EryP。EryP的发育和成熟仍然没有明确的定义。我们对不同阶段的胚胎血液的分析表明，从E9.5到E12.5，EryP血统有一个逐步发育的过程。此后，Eryd也出现在血流中，这两种血统很难区分。我们已经建立了一个转基因小鼠系，其中人类epsilon-珠蛋白基因启动子驱动绿色荧光蛋白的表达仅在EryP谱系内。在这里，我们使用这条线来描述EryP成熟时细胞形态和表面标记表达的变化，并跟踪EryP数量和整个妊娠过程中的去核。这项研究确定了先前未知的同步发育阶段，导致小鼠胚胎中EryPs的成熟。出乎意料的是，我们发现EryPs是一个稳定的细胞群体，一直持续到妊娠结束。
Primitive erythroblasts (EryPs) are the first hematopoietic cell type to form during mammalian embryogenesis and emerge within the blood islands of the yolk sac. Large, nucleated EryPs begin to circulate around midgestation, when connections between yolk sac and embryonic vasculature mature. Two to 3 days later, small cells of the definitive erythroid lineage (EryD) begin to differentiate within the fetal liver and rapidly outnumber EryPs in the circulation. The development and maturation of EryPs remain poorly defined. Our analysis of embryonic blood at different stages reveals a stepwise developmental progression within the EryP lineage from E9.5 to E12.5. Thereafter, EryDs are also present in the bloodstream, and the 2 lineages are not easily distinguished. We have generated a transgenic mouse line in which the human epsilon-globin gene promoter drives expression of green fluorescent protein exclusively within the EryP lineage. Here, we have used this line to characterize changes in cell morphology and surface-marker expression as EryPs mature and to track EryP numbers and enucleation throughout gestation. This study identifies previously unrecognized synchronous developmental stages leading to the maturation of EryPs in the mouse embryo. Unexpectedly, we find that EryPs are a stable cell population that persists through the end of gestation.