Effect of neem limonoids on lactate dehydrogenase (LDH) of the rice leaffolder, Chaphalocrocis medinalis (Guenee) (Insecta: Lepidoptera: Pyralidae)

Effect of neem limonoids on lactate dehydrogenase (LDH) of the rice leaffolder, Chaphalocrocis medinalis (Guenee) (Insecta: Lepidoptera: Pyralidae)
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DOI:
10.1016/j.chemosphere.2005.07.009
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发表时间:
2006-03-01
期刊:
影响因子:
8.8
通讯作者:
Murugan, K
Murugan, K
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
Nathan, SS;Kalaivani, K;Murugan, K

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印楝源自印楝树 Azadirachta indica A. Juss。 (楝科),其主要杀虫成分是四降三萜类印苦楝素和其他柠檬苦素类化合物。研究了印楝柠檬苦素类印苦楝素、萨兰宁、脱乙酰基杜宁、盖杜宁、17-羟基氮杂二酮和脱乙酰宁宾对稻纵卷叶螟(RLF)稻纵卷叶螟(鳞翅目:螟科)幼虫乳酸脱氢酶(LDH)活性的影响。在所有测试浓度下,酶活性相对于对照有所降低。在生物测定中,当饲喂用印楝柠檬苦素处理过的稻叶饲料时,稻纵卷叶螟幼虫的肠道组织酶和 LDH 水平会受到影响。这些结果表明印楝柠檬苦素影响 LDH 活性。这些影响在早期幼虫中最为明显。在所有实验中,印楝素是所有柠檬苦素中最有效的,表明具有很强的酶抑制作用。关于 LDH 活性建立了明确的剂量-反应关系。 (c) 2005 年,爱思唯尔有限公司出版。
Neem is derived from the neem tree Azadirachta indica A. Juss. (Meliaceae), and its primary insecticidal component is the tetranortriterpenoid azadirachtin and other limonoids. The effect of neem limonoids azadirachtin, salannin, deacetylgedunin, gedunin, 17-hydroxyazadiradione and deacetylnimbin on enzyme lactate dehydrogenase (LDH) activity of the rice leaffolder (RLF) Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) larvae was investigated. There was a decrease in enzyme activity relative to the control at all concentrations tested. When fed a diet of rice leaves treated with neem limonoids in bioassays, gut tissue enzyme, LDH levels in rice leaffolder larvae are affected. These results indicate neem limonoids affect LDH activity. These effects are most pronounced in early instar larvae. Azadirachtin was the most potent in of all the limonoids in all experiments indicating strong enzyme inhibition. Clear dose-response relationships were established with respect to LDH activity. (c) 2005 Published by Elsevier Ltd.