GAIT-STANCE DURATION AS A MEASURE OF INJURY AND RECOVERY IN THE RAT SCIATIC-NERVE MODEL
GAIT-STANCE DURATION AS A MEASURE OF INJURY AND RECOVERY IN THE RAT SCIATIC-NERVE MODEL
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DOI：
10.1016/0165-0270(94)90054-x
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发表时间：
1994-04-01
影响因子：
3
通讯作者：
SISKEN, BF
中科院分区：
文献类型：
--
作者：
WALKER, JL;EVANS, JM;SISKEN, BF
大鼠坐骨神经是研究周围神经挤压伤的良好动物模型。在该模型中，足迹分析是测量损伤后功能恢复的最广泛使用的非侵入性方法。然而，这种方法有很大的局限性，因为无法获得清晰的可重现指纹，特别是当损伤严重时，并且这些指纹随着步态速度的变化而变化。在肌挛缩或脚趾丢失的情况下，足迹分析是不可靠的。我们描述了一种新的技术，步态持续时间，它能够非侵入性地量化大鼠模型的功能恢复。这种方法不依赖于步态中准确的脚部定位。它利用动物行走的视频记录，并通过计算经过的帧的数量来测量每只后脚接触地板的时间。通过配对连续的步态，它将速度变化引起的变化降至最低，并通过计算受伤/未受伤后脚的比率，可以与正常步态进行比较。这种方法显示了类似于足迹分析的恢复模式，动物间变异性很小。我们认为，与足迹分析相比，它在测量大鼠坐骨神经损伤模型中的功能恢复方面具有显著的优势。
The rat sciatic nerve is a well-established animal model for the study of peripheral nerve crush injury. Footprint analysis is the most widely used non-invasive method of measuring functional recovery after injury in this model. However, this method has significant limitations due to inability to obtain clear reproducible prints, especially when the injury is severe, and variation of these prints with gait velocity. In the case of contracture or toe loss, footprint analysis is unreliable. We describe a new technique, gait-stance duration, which is capable of non-invasively quantitating functional recovery in the rat model. This method is not dependent on accurate foot positioning during gait. It utilizes video recording of the animal walking and measures the time each hind foot is in contact with the floor by counting the number of frames that pass. By pairing consecutive steps, it minimizes variation due to changes in velocity and, by calculating a ratio of injured/uninjured hind feet, comparisons to normal gait can be made. This method shows recovery patterns similar to footprint analysis with small inter-animal variability. We believe it has significant advantages over footprint analysis for the measurement of functional recovery in the crushed sciatic nerve rat model.