VASCULAR ENDOTHELIAL GROWTH-FACTOR IN OCULAR FLUID OF PATIENTS WITH DIABETIC-RETINOPATHY AND OTHER RETINAL DISORDERS
VASCULAR ENDOTHELIAL GROWTH-FACTOR IN OCULAR FLUID OF PATIENTS WITH DIABETIC-RETINOPATHY AND OTHER RETINAL DISORDERS
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DOI：
10.1056/nejm199412013312203
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发表时间：
1994-12-01
影响因子：
158.5
通讯作者：
KING, GL
中科院分区：
文献类型：
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作者：
AIELLO, LP;AVERY, RL;KING, GL
背景视网膜缺血诱导眼内新生血管形成，这通常导致青光眼、玻璃体出血和视网膜脱离，推测是通过刺激血管生成分子的释放。血管内皮生长因子（VEGF）是一种内皮细胞特异性的血管生成因子，缺氧时其产生增加。我们测量了VEGF的浓度在210个样本的眼内液从164例接受眼内手术，使用放射免疫分析和放射受体测定。用体外视网膜内皮细胞生长试验和VEGF中和抗体测定玻璃体增殖能力。VEGF在136例糖尿病视网膜病变患者的眼液标本中有69例阳性，在38例虹膜新生血管患者的眼液标本中有29例阳性，在4例视网膜中央静脉缺血性阻塞患者的眼液标本中有3例阳性，而在31例无新生血管病变患者的眼液标本中有2例阳性（P
Background. Retinal ischemia induces intraocular neovascularization, which often leads to glaucoma, vitreous hemorrhage, and retinal detachment, presumably by stimulating the release of angiogenic molecules. Vascular endothelial growth factor (VEGF) is an endothelial-cell-specific angiogenic factor whose production is increased by hypoxia.Methods. We measured the concentration of VEGF in 210 specimens of ocular fluid obtained from 164 patients undergoing intraocular surgery, using both radioimmunoassays and radioreceptor assays. Vitreous proliferative potential was measured with in vitro assays of the growth of retinal endothelial cells and with VEGF-neutralizing antibody.Results. VEGF was detected in 69 of 136 ocular-fluid samples from patients with diabetic retinopathy, 29 of 38 samples from patients with neovascularization of the iris, and 3 of 4 samples from patients with ischemic occlusion of the central retinal vein, as compared with 2 of 31 samples from patients with no neovascular disorders (P