A HIGHLY RESOLVED, OXYGEN-EVOLVING PHOTOSYSTEM-II PREPARATION FROM SPINACH THYLAKOID MEMBRANES - ELECTRON-PARAMAGNETIC-RES AND ELECTRON-TRANSPORT PROPERTIES
A HIGHLY RESOLVED, OXYGEN-EVOLVING PHOTOSYSTEM-II PREPARATION FROM SPINACH THYLAKOID MEMBRANES - ELECTRON-PARAMAGNETIC-RES AND ELECTRON-TRANSPORT PROPERTIES
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DOI：
10.1016/0014-5793(81)80608-4
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发表时间：
1981-01-01
期刊：
影响因子：
3.5
通讯作者：
YOCUM, CF
中科院分区：
文献类型：
--
作者：
BERTHOLD, DA;BABCOCK, GT;YOCUM, CF
叶绿体类囊体膜的光系统II提供光产生的氧化等效物，最终将水氧化为氧气。已知该系统包含一个反应中心的叶绿素复合体P680，一个记为Q的苯醌受体，一个记为Z的P680供体分子和一个高潜力的细胞色素b-559[L]。到目前为止，这些成分与被认为是水分裂实际位置的推测的锰蛋白之间的关系尚不清楚。试图通过洗涤剂的作用从高等植物叶绿体的完整膜上解决这种光反应，已经导致了从反应中心[2，3]到更复杂的具有泡状结构的组件的各种准备[3]。虽然这些制剂通常能够支持从添加的供体到外源受体的电子转移，但已报道的所有高等植物制剂都无法进行高活性的放氧。光系统II可以从蓝藻Phormidium laminosum的膜中分离出来[4]。与高等植物的那些制剂相比，这些制剂保留了高放氧率[4]，并富含EPR信号II和PS I的残留污染，如一些信号I(P700‘)[5]的存在所表明的那样。膜；在这里，我们报告了通过洗涤剂分解叶绿体类囊体膜而获得的放氧PS II制剂的性质。
Photosystem II of chloroplast thylakoid membranes provides light-generated oxidizing equivalents which ultimately oxidize water to oxygen. The system is known to contain a reaction center chlorophyll complex, P680, a quinone acceptor denoted as Q, a P680 donor molecule designated as Z and a highpotential cytochrome b-559 [l]. The relationship between these components and the presumed manganese protein thought to be the actual site of watersplitting is as yet poorly understood. Attempts to resolve this photoreaction from intact membranes of higher plant chloroplasts by detergent action have resulted in a variety of preparations ranging from reaction centers [2, 3] to more complex assemblies with vesicular structure [3]. While these preparations are generally able to support electron transfer from an added donor to an exogenous acceptor, all higher plant preparations reported are unable to carry out oxygen evolution with high activity. Photosystem II may be isolated from membranes of the cyanobacterium Phormidium laminosum [4]. These preparations, in contrast to those from higher plants, retain high rates of oxygen evolution [4] and are enriched in EPR signal II with residual contamination from PS I, as shown by the presence of some signal I (P700’)[5]. membranes; here we report the properties of oxygenevolving PS II preparations obtained by detergent resolution of chloroplast thylakoid membranes.