Spectral and time-resolved fluorescence signature of four weed species as affected by selected herbicides
Spectral and time-resolved fluorescence signature of four weed species as affected by selected herbicides
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DOI：
10.1016/j.pestbp.2011.07.002
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发表时间：
2011-09-01
影响因子：
4.7
通讯作者：
Noga, Georg
中科院分区：
文献类型：
--
作者：
Hunsche, Mauricio;Buerling, Kathrin;Noga, Georg
In the present study we show for the first time the suitability of the laser-induced fluorescence technique to evaluate in vivo herbicide-induced damages as revealed by changes of fluorescence spectra and life-time. Four herbicides of different modes of action (glyphosate, bromoxynil, mesotrione, and amitrole) were selected and applied to four weed species (Stellaria media, Setaria viridis, Cheno podium album, and Viola arvensis). Modifications of the fluorescence signature depended on the agrochemical - plant species interaction as well as on the time after application. Measurements in the red and far-red spectral region reveal disturbances in the functionality of the photosynthetic apparatus and chlorophyll concentration, e.g. after application of bromoxynil or mesotrione. Recordings in the blue and green spectral regions indicate changes of both amount and composition of specific fluorophores, i.e. after application of glyphosate and amitrole. In all spectral measurements, the position of peak maxima was not affected by herbicide application. The fluorescence lifetime, expressed as LTmean or as lifetime 1 (LT1, short-duration) and LT2 (long-duration) fractions, provided additional information to the spectrally resolved data. Thereby, significant alterations of the lifetime duration and fractional characteristics were observed at specific wavelengths, e.g. after application of bromoxynil, mesotrione, or amitrole. (C) 2011 Elsevier Inc. All rights reserved.
