INCREASED SUPEROXIDE PRODUCTION BY MONONUCLEAR-CELLS OF PATIENTS WITH HYPERTRIGLYCERIDEMIA AND DIABETES
INCREASED SUPEROXIDE PRODUCTION BY MONONUCLEAR-CELLS OF PATIENTS WITH HYPERTRIGLYCERIDEMIA AND DIABETES
复制标题
DOI：
10.2337/diabetes.37.6.832
复制
发表时间：
1988-06-01
期刊：
影响因子：
7.7
通讯作者：
ARIMORI, S
中科院分区：
文献类型：
--
作者：
HIRAMATSU, K;ARIMORI, S
糖尿病合并高血糖患者常发生动脉粥样硬化。由于超氧化物（O2-）被怀疑在动脉粥样硬化的起始中起重要作用，我们研究了糖尿病和高脂血症患者的循环单核细胞是否产生异常量的O2-。当细胞被4 β-O2刺激时，测量超氧化物歧化酶-可氧化O2-的产生速率。佛波醇12 β-肉豆蔻酸酯13 α-乙酸盐（PMA）或调理酵母聚糖（OZ）。此外，从其他三个组（正常，单纯糖尿病，单纯hyperglyceridemic）的单核细胞的O2-生产率进行了测定。我们发现糖尿病高血糖组单核细胞产生O2-的速率显著高于正常组、糖尿病组和高血糖组。当单个核细胞的O2-产生速率与所有受试个体的血浆甘油三酯水平作图时，它们呈正相关（PMA刺激r = 0.73，OZ刺激r = 0.79，P <0.01）。然而，O2-产生速率与其他参数无关，即，血浆胆固醇水平、红细胞中血红蛋白A1水平和单核细胞中游离胆固醇与磷脂的摩尔比。因此，我们得出结论，观察到的糖尿病高血糖单核细胞中的O2-产生率升高是高血糖状态的反映，并不是糖尿病高血糖状态所独有的。此外，O2-可能参与糖尿病高脂血症患者动脉粥样硬化的发病机制时，动脉粥样硬化的糖尿病特异性致动脉粥样硬化因子伴随存在。
Diabetic patients with hypertriglyceridemia frequently develop atherosclerosis. Because superoxide (O2-) is suspected to play an important role in the initiation of atherosclerosis, we investigated whether an abnormal amount of O2- was produced by circulating mononuclear cells of patients with both diabetes mellitus and hypertriglyceridemia. The rate of production of superoxide dismutase-inhibitable O2- was measured when cells were stimulated by either 4.beta.-phorbol 12.beta.-myristate 13.alpha.-acetate (PMA) or by opsonized zymosan (OZ). In addition, the rates of O2- production by mononuclear cells drawn from three other groups (normal, solely diabetic, and solely hypertriglyceridemic) were determined. We found that the rate of O2- production by mononuclear cells from the diabetic hypertriglyceridemic group was significantly higher than that from normal, diabetic, and hypertriglyceridemic groups. When the rates of O2- production by mononuclear cells were plotted against the levels of plasma triglyceride for all individuals tested, they correlated positively (r = .73 in PMA stimulation and r = .79 in OZ stimulation, P < .01). However, the rate of O2- production did not relate to other parameters, i.e., plasma cholesterol level, hemoglobin A1 level in erythrocytes, and the molar ratio of free cholesterol to phospholipid in mononuclear cells. Thus, we conclude that the observed elevated rate of O2- production in the diabetic hypertriglyceridemic mononuclear cells was a reflection of a hypertriglyceridemic condition and was not unique to the diabetic hypertriglyceridemic condition. Also, O2- may be involved in the pathogenesis of atherosclerosis in diabetic hypertriglyceridemic patients when atherogenic factors specific to diabetes are concomitantly present.